Gold nanoparticle probe-based immunoassay as a new tool for tetrodotoxin detection in puffer fish tissues
Gold nanoparticle probe-based immunoassay as a new tool for tetrodotoxin detection in puffer fish tissues
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基于金纳米颗粒探针的免疫分析作为河豚毒素检测的新工具
DOI：
10.1016/j.snb.2010.02.049
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发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
Jun-hui Zhang
中科院分区：
文献类型：
--
作者：
Yu Zhou;Zeng-shan Liu;Yan-song Li;Shi-ying Lu;Hong-lin Ren;Zhao-hui Li;Yuan-yuan Zhang;Feng-guang Pan;Wen-sen Liu;Jun-hui Zhang
建立了一种基于纳米金标记单抗(MAb)的竞争模板免疫分析方法，用于河豚组织中河豚毒素(TTX)的快速检测。在该试验中，使用了两种抗体。样品垫中提供的第一抗体作为检测试剂，固定在诊断硝酸纤维素膜上的第二抗体作为捕捉剂。组织样品中的TTX与固定化TTX-BSA竞争结合到金纳米颗粒标记的单抗上。流动络合物(金纳米颗粒-单抗-TTX)可被二抗捕获，但不能被TTX-BSA(测试线)捕获。测试线的色密度与样品中河豚毒素的浓度在40-8000ngmL−1范围内呈正相关。添加河豚毒素的样品的视觉检测下限为40ngmL−1。基于对河豚毒素检测结果的视觉评价进行定性检测所需的时间小于10min，并且不需要任何设备。该方法可作为TTX现场快速检测的筛选方法。
A competitive format immunoassay based on a gold nanoparticle-labelled monoclonal antibody (Mab) for the rapid detection of tetrodotoxin (TTX) in puffer fish tissues was developed. In the assay, two antibodies were employed. The first antibody supplied in sample pad was as detector reagent and the secondary antibody immobilized on a diagnostic cellulose nitrate membrane was as capture reagent. TTX in tissue sample competed with immobilized TTX–BSA to bind with gold nanoparticle-labelled Mab. The mobile complex (gold nanoparticle–Mab–TTX) can be captured by the secondary antibody but cannot be captured by TTX–BSA (test line). The color density of the test line correlated with the concentration of TTX in sample in the range 40–8000ngmL−1. The visual detection limit of TTX-spiked samples was found to be 40ngmL−1. The assay time of the qualitative test based on the visual evaluation of results for TTX detection was less than 10min, and the assay performance did not require any equipment. This method can be applied as a screening method for rapid testing of TTX on site.
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DOI：
10.1016/j.jchromb.2008.06.030
发表时间：
2008-08-01
影响因子：
3
作者：
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DOI：
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DOI：
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发表时间：
2007-12-01
影响因子：
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