Cellular adaptation contributes to calorie restriction-induced preservation of skeletal muscle in aged rhesus monkeys.
Cellular adaptation contributes to calorie restriction-induced preservation of skeletal muscle in aged rhesus monkeys.
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细胞适应有助于卡路里限制诱导的老年恒河猴骨骼肌保存。
DOI：
10.1016/j.exger.2011.12.009
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发表时间：
2012-03
影响因子：
3.9
通讯作者：
Anderson, Rozalyn M.
中科院分区：
文献类型：
--
作者：
McKiernan, Susan H.;Colman, Ricki J.;Aiken, Erik;Evans, Trent D.;Beasley, T. Mark;Aiken, Judd M.;Weindruch, Richard;Anderson, Rozalyn M.
我们之前已经证明，减少30%的卡路里摄入量的饮食延迟了~16岁至~22岁的成年猴肌肉质量损失的发生，并防止了衰老的多种细胞表型。在本研究中，我们显示了长期（~17年）热量限制（CR）对非常老的猴子（27- 33岁）肌肉老化的影响，并与年龄匹配的对照组猴子自由进食进行了比较，并在整个纵向研究的背景下描述了这些数据。与年龄匹配的对照组相比，在非常老的热量限制（CR）猴中保留了肌肉质量。免疫组织化学分析显示，对照组动物VL中I型纤维的比例随年龄增加而增加，CR可防止这种增加。与早期时间点（16-22岁）相比，老年CR动物的II型纤维横截面积（CSA）减少;然而，CR动物的CSA总损失仅为15%，而在约27岁时，老年对照动物的CSA总损失为36%。两组I型纤维均未检测到萎缩。值得注意的是，在约27岁时，CR动物VL中的I型纤维CSA是对照动物的约1.6倍。在对照组和CR组之间，存在线粒体电子传递系统酶（ETSab）缺陷、细胞色素c氧化酶和高反应性琥珀酸脱氢酶缺失的VL肌纤维的频率相同。我们描述了ETSab纤维CSA的变化，并确定CR纤维对线粒体缺陷的挑战有不同的反应。完整的股直肌肌纤维计数显示，肌纤维密度保存在老年CR动物。我们认为，CR动物的肌纤维比对照动物更能承受和适应肌肉质量的变化。
We have previously shown that a 30% reduced calorie intake diet delayed the onset of muscle mass loss in adult monkeys between ~16 and ~22 years of age and prevented multiple cellular phenotypes of aging. In the present study we show the impact of long term (~17 years) calorie restriction (CR) on muscle aging in very old monkeys (27–33yrs) compared to age-matched Control monkeys fed ad libitum, and describe these data in the context of the whole longitudinal study. Muscle mass was preserved in very old calorie restricted (CR) monkeys compared to age-matched Controls. Immunohistochemical analysis revealed an age-associated increase in the proportion of Type I fibers in the VL from Control animals that was prevented with CR. The cross sectional area (CSA) of Type II fibers was reduced in old CR animals compared to earlier time points (16–22 years of age); however, the total loss in CSA was only 15% in CR animals compared to 36% in old Controls at ~27 years of age. Atrophy was not detected in Type I fibers from either group. Notably, Type I fiber CSA was ~1.6 fold greater in VL from CR animals compared to Control animals at ~27 years of age. The frequency of VL muscle fibers with defects in mitochondrial electron transport system enzymes (ETSab), the absence of cytochrome c oxidase and hyper-reactive succinate dehydrogenase, were identical between Control and CR. We describe changes in ETSab fiber CSA and determined that CR fibers respond differently to the challenge of mitochondrial deficiency. Fiber counts of intact rectus femoris muscles revealed that muscle fiber density was preserved in old CR animals. We suggest that muscle fibers from CR animals are better poised to endure and adapt to changes in muscle mass than those of Control animals.
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DOI：
10.1126/science.1173635
发表时间：
2009-07-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colman RJ;Anderson RM;Johnson SC;Kastman EK;Kosmatka KJ;Beasley TM;Allison DB;Cruzen C;Simmons HA;Kemnitz JW;Weindruch R
通讯作者：
Weindruch R
DOI：
10.1067/mlc.2001.113504
发表时间：
2001-04-01
期刊：
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
影响因子：
--
作者：
Morley, JE;Baumgartner, RN;Nair, KS
通讯作者：
Nair, KS
DOI：
10.1111/j.1749-6632.2002.tb02111.x
发表时间：
2002-01-01
期刊：
INCREASING HEALTHY LIFE SPAN: CONVENTIONAL MEASURES AND SLOWING THE INNATE AGING PROCESS
影响因子：
--
作者：
Aiken, J;Bua, E;McKiernan, S
通讯作者：
McKiernan, S
影响因子：
3.9
作者：
McKiernan, Susan H.;Colman, Ricki J.;Lopez, Marisol;Beasley, T. Mark;Aiken, Judd M.;Anderson, Rozalyn M.;Weindruch, Richard
通讯作者：
Weindruch, Richard
DOI：
10.1139/h01-006
发表时间：
2001-02-01
期刊：
CANADIAN JOURNAL OF APPLIED PHYSIOLOGY-REVUE CANADIENNE DE PHYSIOLOGIE APPLIQUEE
影响因子：
--
作者：
Roubenoff, R
通讯作者：
Roubenoff, R