Consistent removal of hair cells in vestibular end organs by time-dependent transtympanic administration of gentamicin in guinea pigs
Consistent removal of hair cells in vestibular end organs by time-dependent transtympanic administration of gentamicin in guinea pigs
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通过时间依赖性经鼓膜给予豚鼠庆大霉素一致去除前庭终末器官中的毛细胞
DOI：
10.1016/j.jneumeth.2020.109049
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发表时间：
2021
影响因子：
3
通讯作者：
Kakehata Seiji
中科院分区：
文献类型：
--
作者：
Chiba Makoto;Ito Tsukasa;Shinkawa Chikako;Koizumi Yutaka;Hull Melinda;Kakehata Seiji
BackgroundVestibularhaircell loss及其在平衡失调中的作用尚未完全了解，主要是由于缺乏精确的haircell damage protocols.New methodOur damage protocol旨在选择性地去除I型毛细胞，以产生一致和可预测的病变，可用于可靠的动物间和组间比较平衡研究。这一目标是通过在固定的时间段内经鼓膜在圆窗膜和卵圆窗上注射庆大霉素，然后彻底wash.ResultsWe实现了几乎完全和一致的损失，I型毛细胞在94%的外半规管（LSC）壶腹嵴和86%的椭圆囊黄斑，II型毛细胞的损失可以忽略不计，为4%椭圆囊斑的阳性率为6%。虽然前庭功能受到损害的相关研究组，这组有一个零死亡率，没有显着抑制体重gainsComparison与现有methodsGentamicin通常是通过腹腔内全身注射，或最近，transstympanic注射。腹腔内注射方法简单，但死亡率高。经鼓膜注射法产生耳毒性损伤，但病变大小不一致。这种不一致性，防止可靠的比较among animals.ConclusionsThis协议采用transstympanic注射方法，选择性地针对I型毛细胞去除在前庭上皮中的时间依赖性的方式，均匀损害前庭功能，并导致均匀的毛细胞损失。
BackgroundVestibular hair cell loss and its role in balance disorders are not yet completely understood due largely to the lack of precise hair cell damage protocols.New methodOur damage protocol aims to selectively remove type I hair cells in a way that produces consistent and predictable lesions that can be used for reliable inter-animal and inter-group comparison in balance research. This objective is achieved by transtympanic injection of gentamicin on both the round window membrane and oval window over a fixed time period followed by thorough washing.ResultsWe achieved nearly total and consistent loss of type I hair cells at 94 % for the crista ampullaris of the lateral semicircular canal (LSC) and 86 % for the utricular macula with negligible loss of type II hair cells at 4% for the crista ampullaris of the LSC and 6% for the utricular macula. While the vestibular function was compromised in the relevant study group, this group had a zero mortality rate with no significant suppression of body weight gain.Comparison with existing methodsGentamicin is typically administered via intraperitoneal systemic injection or, more recently, transtympanic injection. The intraperitoneal method is simple, but mortality rate is high. The transtympanic injection method produces ototoxic damage but with inconsistent lesion size. This inconsistency prevents reliable comparisons among animals.ConclusionsThis protocol employs a transtympanic injection method which selectively targets type I hair cells for removal in the vestibular epithelia in a time-dependent manner, uniformly damages vestibular function, and causes uniform hair cell loss.
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影响因子：
5
作者：
H. Shimogori;Tsuyoshi Takemoto;T. Mikuriya;H. Yamashita
通讯作者：
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影响因子：
1.4
作者：
DEGROOT, JCMJ;HUIZING, EH;VELDMAN, JE
通讯作者：
VELDMAN, JE
影响因子：
2.5
作者：
Hirvonen, TP;Minor, LB;Carey, JP
通讯作者：
Carey, JP
DOI：
10.1007/s10162-007-0093-8
发表时间：
2007-12-01
影响因子：
2.4
作者：
Lyford-Pike, Sofia;Vogelheim, Casey;Carey, John P.
通讯作者：
Carey, John P.
影响因子：
2.8
作者：
Nakagawa, T;Kim, TS;Ito, J
通讯作者：
Ito, J