Bifidobacterium adolescentis (DSM 20083) and Lactobacillus casei (Lafti L26-DSL): Probiotics Able to Block the In Vitro Adherence of Rotavirus in MA104 Cells.
Bifidobacterium adolescentis (DSM 20083) and Lactobacillus casei (Lafti L26-DSL): Probiotics Able to Block the In Vitro Adherence of Rotavirus in MA104 Cells.
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DOI：
10.1007/s12602-017-9277-7
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发表时间：
2018-03
影响因子：
4.9
通讯作者：
Gutierrez-Fernandez MF
中科院分区：
文献类型：
--
作者：
Fernandez-Duarte KP;Olaya-Galán NN;Salas-Cárdenas SP;Lopez-Rozo J;Gutierrez-Fernandez MF
轮状病毒是全球五岁以下儿童胃肠炎的主要原因。尽管有一些可用的疫苗可以预防这种疾病，但对抗轮状病毒感染引起的腹泻的策略有限。因此，研究人员不断寻找其他通过益生菌控制腹泻的方法。为了证明一些益生菌在体外干扰 MA104 细胞轮状病毒感染的能力，乳酸菌菌株。和双歧杆菌属。在病毒感染前在 MA104 细胞中进行了测试。作为初步测定，用活细菌进行阻断效应处理。在此筛选试验中，最初十种细菌中有四种显示病毒感染略有减少（以感染百分比衡量）。干酪乳杆菌 (Lafti L26-DSL)、发酵乳杆菌 (ATCC 9338)、青春双歧杆菌 (DSM 20083) 和两歧双歧杆菌 (ATCC 11863) 用于进一步实验。为了评估从上述细菌获得的基于蛋白质的代谢物，测试了三种不同的处理方法：（i）在病毒感染前细胞暴露于基于蛋白质的代谢物，（ii）在病毒感染后暴露于基于蛋白质的代谢物，以及（iii）在病毒感染细胞培养物之前将病毒与基于蛋白质的代谢物共孵育。在将病毒和基于蛋白质的代谢物添加到细胞培养物中之前，在病毒和基于蛋白质的代谢物的共孵育测定过程中观察到基于蛋白质的代谢物所表现出的最佳效果。结果显示，干酪乳杆菌和青春双歧杆菌存在时，感染率分别为 25% 和 37%。这些结果表明，抗病毒作用可能直接与病毒颗粒发生，而不是对病毒进入所需的细胞受体产生阻断作用。
Rotavirus is the leading worldwide cause of gastroenteritis in children under five years of age. Even though there are some available vaccines to prevent the disease, there are limited strategies for challenging diarrhea induced by rotavirus infection. For this reason, researchers are constantly searching for other approaches to control diarrhea by means of probiotics. In order to demonstrate the ability of some probiotic bacteria to interfere with the in vitro rotavirus infection in MA104 cells, strains of Lactobacillus sp. and Bifidobacterium sp. were tested in MA104 cells before the viral infection. As a preliminary assay, a blocking effect treatment was performed with viable bacteria. In this screening assay, four of initial ten bacteria showed a slight reduction of the viral infection (measured by percentage of infection). L. casei (Lafti L26-DSL), L. fermentum(ATCC 9338), B. adolescentis (DSM 20083), and B. bifidum (ATCC 11863) were used in further experiments. Three different treatments were tested in order to evaluate protein-based metabolites obtained from mentioned bacteria: (i) cell exposure to the protein-based metabolites before viral infection, (ii) exposure to protein-based metabolites after viral infection, and (iii) co-incubation of the virus and protein-based metabolites before viral infection to the cell culture. The best effect performed by protein-based metabolites was observed during the co-incubation assay of the virus and protein-based metabolites before adding them into the cell culture. The results showed 25 and 37% of infection in the presence of L. casei and B. adolescentis respectively. These results suggest that the antiviral effect may be occurring directly with the viral particle instead of making a blocking effect of the cellular receptors that are needed for the viral entrance.
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影响因子：
2.4
作者：
Salazar-Lindo E;Miranda-Langschwager P;Campos-Sanchez M;Chea-Woo E;Sack RB
通讯作者：
Sack RB
影响因子：
2.9
作者：
Farthing, Michael;Salam, Mohammed A.;LeMair, Anton
通讯作者：
LeMair, Anton
影响因子：
3.6
作者：
Chen, Chien-Chang;Kong, Man-Shan;Lin, Tzou-Yien
通讯作者：
Lin, Tzou-Yien
DOI：
10.1016/j.ijfoodmicro.2006.10.044
发表时间：
2007-04-10
影响因子：
5.4
作者：
Botic, Tanja;Klingberg, Trine Dano;Cencic, Avrelija
通讯作者：
Cencic, Avrelija
影响因子：
2.9
作者：
Huang, Yung-Feng;Liu, Po-Yen;Chen, Kow-Tong
通讯作者：
Chen, Kow-Tong