Effects of the time dependence of a bioluminescent source on the tomographic reconstruction.
Effects of the time dependence of a bioluminescent source on the tomographic reconstruction.
复制标题
生物发光源的时间依赖性对断层扫描重建的影响。
作者：
Mehmet Burcin Unlu;G. Gulsen
在该模拟研究中有两个目标：（1）表明生物发光源的时间变化可能导致断层图像中的伪影，使得量化和定位变得不可能;以及（2）表明光动力学的先验知识可以用于消除这些伪影。这些目标的动机是，荧光素酶的半衰期已被报道为30分钟至2小时在体内的事实。我们进行二维模拟。我们考虑一个直径为40 mm的圆形区域，其中直径为6 mm的夹杂物位于距离中心10 mm处。使用基于有限元的正向求解器模拟测量数据。我们对非接触测量进行建模，以便从四个90度的视图收集四个波长的数据。结果表明，总成像时间与指数衰减生物光源半衰期的比值是光源重建的决定性因素。它还表明，需要进行断层生物发光成像的短半衰期的生物发光源和空间先验信息的使用是不够的源动力学的先验知识。
There are two goals in this simulation study: (1) to show that the time variation of the bioluminescence source can cause artifacts in the tomographic images such that quantification and localization becomes impossible; and (2) to show that the a priori knowledge of the light kinetics can be used to eliminate these artifacts. These goals are motivated by the fact that the half-life of luciferase has been reported as 30 min to 2 h in vivo. We perform two-dimensional simulations. We consider a 40 mm diameter circular region with an inclusion of 6 mm diameter located 10 mm away from the center. The measurement data is simulated using a finite-element-based forward solver. We model the noncontact measurements such that four-wavelength data is collected from four 90 degrees apart views. The results show that the ratio of the total imaging time to the half-life of the exponentially decaying bioluminescent source is the deciding factor in the reconstruction of the source. It is also demonstrated that a priori knowledge of the source kinetics is required to perform tomographic bioluminescence imaging of short half-life bioluminescent sources and the use of spatial a priori information alone is not adequate.
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