Simian varicella virus gene expression during acute and latent infection of rhesus macaques.
Simian varicella virus gene expression during acute and latent infection of rhesus macaques.
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恒河猴急性和潜伏感染期间猿猴水痘病毒基因表达。
DOI：
10.1007/s13365-011-0057-y
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发表时间：
2011-12
影响因子：
3.2
通讯作者：
Messaoudi, Ilhem
中科院分区：
文献类型：
--
作者：
Meyer, Christine;Kerns, Amelia;Barron, Alex;Kreklywich, Craig;Streblow, Daniel N.;Messaoudi, Ilhem
水痘带状疱疹病毒（VZV）是一种嗜神经性α-疱疹病毒，在初次感染时引起水痘，并在感觉神经节中建立潜伏期。VZV的重新激活导致带状疱疹和其他神经系统并发症。我们对免疫活性个体急性和潜伏感染期间VZV转录组的理解仍然不完整。用同源猿猴水痘病毒（SVV）感染恒河猴概括了VZV感染的特征。因此，我们通过定量实时逆转录酶PCR（RT-qPCR）在支气管肺泡灌洗（BAL）细胞和外周血单核细胞（PBMC）的急性感染过程中，以及在从相同的恒河猴获得的感觉神经节潜伏期SVV转录组的特征。在急性感染期间，检测到所有已知的SVV开放阅读框（ORF），并且最丰富表达的ORF参与病毒复制和组装，例如转录激活因子ORF 63和结构蛋白ORF 41和ORF 49。相反，潜伏性SVV基因表达受到高度限制。ORF 61是一种病毒反式激活因子和潜伏期相关的转录物，是感觉神经节中检测到的最普遍的转录物。我们还检测到ORF A、B、4、10、63、64、65、66和68，但频率显著低于ORF 61。这种全面的分析揭示了可能在SVV潜伏期的建立和/或维持中起作用的基因。
Varicella zoster virus (VZV) is a neurotropic α-herpesvirus that causes chickenpox during primary infection and establishes latency in sensory ganglia. Reactivation of VZV results in herpes zoster and other neurological complications. Our understanding of the VZV transcriptome during acute and latent infection in immune competent individuals remains incomplete. Infection of rhesus macaques with the homologous simian varicella virus (SVV) recapitulates the hallmarks of VZV infection. We therefore characterized the SVV transcriptome by quantitative real-time reverse transcriptase PCR (RT-qPCR) during acute infection in bronchial alveolar lavage (BAL) cells and peripheral blood mononuclear cells (PBMC), and during latency in sensory ganglia obtained from the same rhesus macaques. During acute infection, all known SVV open reading frames (ORFs) were detected and the most abundantly expressed ORFs are involved in virus replication and assembly such as the transcriptional activator ORF 63 and the structural proteins ORF 41 and ORF 49. In contrast, latent SVV gene expression is highly restricted. ORF 61, a viral transactivator and latency-associated transcript, is the most prevalent transcript detected in sensory ganglia. We also detected ORFs A, B, 4, 10, 63, 64, 65, 66 and 68 though significantly less frequently than ORF 61. This comprehensive analysis has revealed genes that potentially play a role in the establishment and/or maintenance of SVV latency.
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影响因子：
5.4
作者：
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通讯作者：
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