High prevalence of hpv multiple genotypes in women with persistent chlamydia trachomatis infection.
High prevalence of hpv multiple genotypes in women with persistent chlamydia trachomatis infection.
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DOI：
10.1186/1750-9378-9-30
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发表时间：
2014
影响因子：
3.7
通讯作者：
Comar M
中科院分区：
文献类型：
--
作者：
Seraceni S;De Seta F;Colli C;Del Savio R;Pesel G;Zanin V;D'Agaro P;Contini C;Comar M
沙眼衣原体与HR-HPV类型的相互作用突出了宫颈癌发展的核心作用。本研究的目的是调查HPV感染率和基因型分布的妇女在C。沙眼感染和上皮内病变或恶性肿瘤阴性。对1071份宫颈拭子进行了C.通过真实的时间PCR和ompA基因测序进行基因分型。另外，进行定量真实的时间PCR以评估C.沙眼衣原体Hsp 60编码基因（Ct604部分），与感染的持续状态有关。对C.沙眼阳性女性使用Luminex技术。C. 1071例标本中有53例（4.5%）检出沙眼衣原体感染，其中53%的标本Hsp 60基因表达阳性。C.沙眼衣原体阳性率为60.4%（32/53），其中单一基因型占37.5%，多基因型占62.5%。在C的女性中。在沙眼衣原体慢性感染中，68%为HPV合并感染，79%为多种基因型。值得注意的是，C。与仅感染沙眼衣原体的妇女相比，HPV共感染妇女中沙眼衣原体Hsp 60的表达显著降低。沙眼梭在大多数样本中发现沙眼血清型F，与HPV感染无关。HPV多重感染的高患病率已被发现在年轻女性受感染的C。沙眼慢性感染。这些观察结果表明，CHSP 60 -1的表达，干扰细胞凋亡和细胞衰老途径，可能会促进HPV感染的有利的局部微环境。
Chlamydia trachomatis interaction with HR-HPV types has highlighted a central role in cervical cancer development. The aim of this study was to investigate HPV prevalence and genotypes distribution in women at risk for C. trachomatis infection and negative for intraepithelial lesion or malignancy. 1071 cervical swabs were tested for C. trachomatis by Real Time PCR and genotyping by ompA gene sequencing. Additionally, a quantitative Real time-PCR was performed to assess the expression of the C. trachomatis Hsp60–encoding gene (Ct604 portion), linked to a persistent status of infection. HPV infection and genotypes was investigated in C. trachomatis positive women using Luminex technology. C. trachomatis infection was detected in 53 out of 1071 (4.5%) samples, of which the 53% resulted positive for Hsp60 gene expression. The overall prevalence of HPV infection in C. trachomatis positive samples was of 60.4% (32/53): in 37.5% of samples was present a single genotype, while multiple genotypes infections were found in the 62.5% of them. Among women with a C. trachomatis chronic infection, 68% were HPV co-infected and the 79% showed multiple genotypes. Should be noted that levels of C. trachomatis Hsp60 expression in HPV co-infected women were significantly lower compared to women infected only with C. trachomatis. The C. trachomatis serotype F was found in the majority of samples, independently of HPV infection. A high prevalence of HPV multiple infections have been found in young women affected with a C. trachomatis chronic infection. These observations suggested that the expression of CHSP60-1, interfering with both apoptotic and cellular senescence pathways, may promote a favourable local microenvironment for HPV infection.
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影响因子：
4.4
作者：
Brankatschk, Robert;Bodenhausen, Natacha;Buergmann, Helmut
通讯作者：
Buergmann, Helmut
影响因子：
4
作者：
Contini, Carlo;Grilli, Anastasio;Seraceni, Silva
通讯作者：
Seraceni, Silva
影响因子：
8.8
作者：
Comar, Manola;Monasta, Lorenzo;Zauli, Giorgio
通讯作者：
Zauli, Giorgio
DOI：
10.5402/2011/436936
发表时间：
2011-01-01
期刊：
ISRN obstetrics and gynecology
影响因子：
--
作者：
Mascellino, Maria Teresa;Boccia, Priscilla;Oliva, Alessandra
通讯作者：
Oliva, Alessandra
影响因子：
3.4
作者：
Cuschieri, KS;Cubie, HA;McGoogan, E
通讯作者：
McGoogan, E