Variations in chondrogenesis of human bone marrow-derived mesenchymal stem cells in fibrin/alginate blended hydrogels.
Variations in chondrogenesis of human bone marrow-derived mesenchymal stem cells in fibrin/alginate blended hydrogels.
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DOI：
10.1016/j.actbio.2012.06.028
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发表时间：
2012-10
影响因子：
9.7
通讯作者：
Levenston ME
中科院分区：
文献类型：
--
作者：
Ma K;Titan AL;Stafford M;Zheng Ch;Levenston ME
纤维蛋白和海藻酸盐水凝胶被广泛用于支持骨髓间充质干细胞(BM-MSCs)的软骨形成，用于关节软骨和纤维软骨组织工程，每种材料都有明显的优缺点。为了制备一种兼具两种材料优点的凝胶支架，我们以不同的混合比例制备了纤维蛋白/海藻酸盐共混水凝胶，并评价了其凝胶形态、力学性能及其对BM-MSC软骨形成的支持作用。结果表明，当纤维蛋白/海藻酸盐比例降低时，纤维蛋白结构从均匀的纤维结构转变为相互连接的纤维结构，最后在藻酸盐背景下转变为不连续的岛屿，而藻酸盐结构的趋势则相反。纤维蛋白保持了凝胶的延展性，促进了细胞的增殖，而海藻酸盐提高了凝胶的生物稳定性，更好地支持了糖胺聚糖和II型胶原的产生以及软骨基因的表达。共混凝胶具有介于纤维蛋白和海藻酸盐之间的物理和生物学特性。在所检测的混合物中，FA 40：8(40 mg/mL纤维蛋白原与8 mg/mL海藻酸盐混合)被发现是未来张力驱动的BM-MSC纤维软骨形成研究的最合适的组。由于BM-MSC在混合支架的纤维蛋白和藻酸盐区域的分化似乎不同，本研究还强调了通过操纵支架成分的异质性来发展空间异质性组织的潜力。
Fibrin and alginate hydrogels have been widely used to support chondrogenesis of bone marrow-derived mesenchymal stem cells (BM-MSCs) for articular cartilage and fibrocartilage tissue engineering, with distinct advantages and disadvantages to each material. Attempting to produce a gel scaffold exhibiting beneficial characteristics of both materials, we fabricated fibrin/alginate blended hydrogels at various blend ratios and evaluated the gel morphology, mechanical properties and their support for BM-MSC chondrogenesis. Results show that when the fibrin/alginate ratio decreased, the fibrin architecture transitioned from uniform to interconnected fibrous and finally to disconnected islands against an alginate background, with opposing trends in the alginate architecture. Fibrin maintained gel extensibility and promoted cell proliferation, while alginate improved the gel biostability and better supported glycosaminoglycan and collagen II production and chondrogenic gene expression. Blended gels had physical and biological characteristics intermediate between fibrin and alginate. Of the blends examined, FA 40:8 (40 mg/mL fibrinogen blended with 8 mg/mL alginate) was found to be the most appropriate group for future studies on tension-driven BM-MSC fibrochondrogenesis. As BM-MSC differentiation appeared to vary between fibrin and alginate regions of blended scaffolds, this study also highlighted the potential to develop spatially heterogeneous tissues through manipulating the heterogeneity of scaffold composition.
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影响因子：
2.4
作者：
Le Graverand, MPH;Ou, Y;Rattner, JB
通讯作者：
Rattner, JB
影响因子：
4.1
作者：
Connelly, John T.;Vanderploeg, Eric J.;Levenston, Marc E.
通讯作者：
Levenston, Marc E.
影响因子：
4.1
作者：
GROVER, J;ROUGHLEY, PJ
通讯作者：
ROUGHLEY, PJ
影响因子：
14
作者：
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通讯作者：
Burdick, Jason A.
DOI：
10.1002/jbm.b.30053
发表时间：
2004-08-15
影响因子：
3.4
作者：
Leddy, HA;Awad, HA;Guilak, F
通讯作者：
Guilak, F