Nicotinic Acetylcholine Receptor α9 and α10 Subunits Are Expressed in the Brain of Mice.
Nicotinic Acetylcholine Receptor α9 and α10 Subunits Are Expressed in the Brain of Mice.
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DOI：
10.3389/fncel.2017.00282
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发表时间：
2017
影响因子：
5.3
通讯作者：
Skok M
中科院分区：
文献类型：
--
作者：
Lykhmus O;Voytenko LP;Lips KS;Bergen I;Krasteva-Christ G;Vetter DE;Kummer W;Skok M
α9和α10烟碱乙酰胆碱受体（nAChR）亚基可能是整个cys-loop配体门控离子通道超家族（包括乙酰胆碱、GABA、甘氨酸和5 -羟色胺嗜离子受体）的进化前体。含有α9和α10亚基的nachr存在于内耳、背根神经节和许多非兴奋性组织中，但其在中枢神经系统中的表达尚未得到明确证实。通过RT-PCR和免疫化学方法，我们发现了α9和α10 nAChR亚基在小鼠脑内的存在，并在α7 - / -、α9 - / -和α10 - / -小鼠脑制剂中表现出选择性。在延髓（MO）、小脑、中脑（MB）、丘脑和壳核（TP）、体感皮层（SC）、额叶皮层（FC）和海马中均发现α9和α10 RNA转录本。ELISA在FC、SC、MO、TP和海马中观察到高α9选择性信号，α10选择性信号在MO和FC中最高。α9和α10蛋白主要存在于脑线粒体中，而α7-、α9-和α10选择性抗体主要染色神经元和肥大星形胶质细胞，未染色小胶质细胞。α9-和α10阳性细胞在小脑和上橄榄（SO）内形成有序的结构或区，并随机分布于FC内α7阳性细胞之间；在海马的CA1、CA3和CA4区均有发现，但在CA2区未发现。α7−/−小鼠α9和α10亚基表达上调，α10−/−小鼠α7和α9亚基表达下调。我们得出结论，α9和α10 nAChR亚基在小鼠脑的不同神经元和脑线粒体中表达，并且在α7亚基缺失的情况下代偿上调。
The α9 and α10 nicotinic acetylcholine receptor (nAChR) subunits are likely to be the evolutionary precursors to the entire cys-loop superfamily of ligand-gated ion channels, which includes acetylcholine, GABA, glycine and serotonin ionotropic receptors. nAChRs containing α9 and α10 subunits are found in the inner ear, dorsal root ganglia and many non-excitable tissues, but their expression in the central nervous system has not been definitely demonstrated. Here we show the presence of both α9 and α10 nAChR subunits in the mouse brain by RT-PCR and immunochemical approaches with a range of nAChR subunit-selective antibodies, which selectivity was demonstrated in the brain preparations of α7−/−, α9−/− and α10−/− mice. The α9 and α10 RNA transcripts were found in medulla oblongata (MO), cerebellum, midbrain (MB), thalamus and putamen (TP), somatosensory cortex (SC), frontal cortex (FC) and hippocampus. High α9-selective signal in ELISA was observed in the FC, SC, MO, TP and hippocampus and α10-selective signal was the highest in MO and FC. The α9 and α10 proteins were found in the brain mitochondria, while their presence on the plasma membrane has not been definitely confirmed The α7-, α9- and α10-selective antibodies stained mainly neurons and hypertrophied astrocytes, but not microglia. The α9- and α10-positive cells formed ordered structures or zones in cerebellum and superior olive (SO) and were randomly distributed among α7-positive cells in the FC; they were found in CA1, CA3 and CA4, but not in CA2 region of the hippocampus. The α9 and α10 subunits were up-regulated in α7−/− mice and both α7 and α9 subunits were down-regulated in α10−/− mice. We conclude that α9 and α10 nAChR subunits are expressed in distinct neurons of the mouse brain and in the brain mitochondria and are compensatory up-regulated in the absence of α7 subunits.
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DOI：
10.1016/j.biocel.2014.01.001
发表时间：
2014-04-01
影响因子：
4
作者：
Gergalova, Galyna;Lykhmus, Olena;Skok, Maryna
通讯作者：
Skok, Maryna
DOI：
10.1165/rcmb.2011-0171oc
发表时间：
2012-01-01
影响因子：
6.4
作者：
Hollenhorst, Monika I.;Lips, Katrin S.;Fronius, Martin
通讯作者：
Fronius, Martin
DOI：
10.1111/j.1749-6632.2002.tb07574.x
发表时间：
2002-01-01
期刊：
CREBELLUM: RECENT DEVELOPMENTS IN CEREBELLAR RESEARCH
影响因子：
--
作者：
Ito, M
通讯作者：
Ito, M
影响因子：
2.5
作者：
Koval, OM;Voitenko, LP;Skok, VI
通讯作者：
Skok, VI
影响因子：
4.3
作者：
Colombini, M
通讯作者：
Colombini, M