Differential effect of early antibiotic intervention on bacterial fermentation patterns and mucosal gene expression in the colon of pigs under diets with different protein levels
Differential effect of early antibiotic intervention on bacterial fermentation patterns and mucosal gene expression in the colon of pigs under diets with different protein levels
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早期抗生素干预对不同蛋白质水平日粮下猪结肠细菌发酵模式和粘膜基因表达的差异影响
DOI：
10.1007/s00253-016-7985-7
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发表时间：
2017-03
影响因子：
5
通讯作者：
Zhu Weiyun
中科院分区：
文献类型：
--
作者：
Zhang Chuanjian;Yu Miao;Yang Yuxiang;Mu Chunlong;Su Yong;Zhu Weiyun
本研究旨在评价早期抗生素干预（EAI）对不同蛋白质水平日粮猪结肠细菌发酵模式和粘膜免疫指标的影响。在第7天（d），给18窝仔猪饲喂不含或含生长促进抗生素的蠕动饲料，直至第42天。在第42天，每组内的猪进一步随机分配到正常或低粗蛋白（CP）饮食。在第77天和第120天，每组屠宰5头猪，用于分析结肠细菌、代谢产物和粘膜基因表达。结果表明，低CP日粮显著提高了77 d时丙酸和丁酸的含量，降低了氨和酚的含量（P< 0.05）。EAI显著提高了77 d时正常CP日粮中对甲酚和吲哚的含量（P< 0.05）。低CP日粮显著影响（P< 0.05）某些细菌群（厚壁菌门、梭菌IV群、梭菌XIVa群、大肠杆菌和乳酸杆菌），但EAI影响有限。低CP饮食下调了促炎细胞因子、Toll样受体（TLR 4）、髓样分化因子88（MyD 88）和核因子-κB p65（NF-κB p65）的基因表达（P< 0.05）。EAI在77 d时显著上调正常CP饲料下IL-8和IFN-γ mRNA的表达（P< 0.05）。此外，E.低CP日粮下大肠杆菌和氨水平与促炎细胞因子基因表达的下调呈正相关，而促炎细胞因子基因表达的下调与TLR 4-MyD 88-NF-κB信号通路的下调呈正相关。总之，EAI在正常CP饮食下具有短期效应，增加芳香族氨基酸发酵和促炎细胞因子的基因表达。低CP饮食显著降低蛋白质发酵，改变微生物群落结构，下调促炎细胞因子基因表达，其机制可能与下调TLR 4-MyD 88-NF-κB信号通路有关。
The study aimed to evaluate the effects of early antibiotic intervention (EAI) on bacterial fermentation patterns and mucosal immune markers in the colon of pigs with different protein level diets. Eighteen litters of piglets at day (d) 7 were fed creep feed without or with growth promoting antibiotics until d 42. At d 42, pigs within each group were further randomly assigned to a normal- or low-crude protein (CP) diet. At d 77 and d 120, five pigs per group were slaughtered for analyzing colonic bacteria, metabolites, and mucosal gene expressions. Results showed that low-CP diet increased propionate and butyrate concentrations at d 77 but reduced ammonia and phenol concentrations (P< 0.05). EAI increasedp-cresol and indole concentrations under normal-CP diet at d 77 (P< 0.05). Low-CP diet significantly affected (P< 0.05) some bacteria groups (Firmicutes,Clostridiumcluster IV,Clostridiumcluster XIVa,Escherichia coli, andLactobacillus), but EAI showed limited effects. Low-CP diet down-regulated gene expressions of pro-inflammatory cytokines, toll-like receptor (TLR4), myeloid differentiating factor 88 (MyD88), and nuclear factor-κB p65 (NF-κB p65) (P< 0.05). EAI up-regulated mRNA expressions of interleukin-8 (IL-8) and interferon-γ (IFN-γ) under normal-CP diet at d 77 (P< 0.05). Furthermore, reductions ofE. coliand ammonia under low-CP diet were positively correlated with down-regulated gene expressions of pro-inflammatory cytokines, which were positively correlated with the down-regulated TLR4-MyD88-NF-κB signaling pathway. In conclusion, EAI had short-term effects under normal-CP diet with increased aromatic amino acid fermentation and gene expressions of pro-inflammatory cytokines. Low-CP diet markedly reduced protein fermentation, modified microbial communities, and down-regulated gene expressions of pro-inflammatory cytokines possibly via down-regulating TLR4-MyD88-NF-κB signaling pathway.
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影响因子：
17.1
作者：
Turnbaugh PJ;Ridaura VK;Faith JJ;Rey FE;Knight R;Gordon JI
通讯作者：
Gordon JI
影响因子：
7.1
作者：
Russell, Wendy R.;Gratz, Silvia W.;Flint, Harry J.
通讯作者：
Flint, Harry J.
小鼠膳食精氨酸补充剂改变微生物群并激活肠道先天免疫
影响因子：
4.2
作者：
Ren, Wenkai;Chen, Shuai;Wu, Guoyao
通讯作者：
Wu, Guoyao
影响因子：
1.9
作者：
RASMUSSEN, HS;HOLTUG, K;MORTENSEN, PB
通讯作者：
MORTENSEN, PB
影响因子：
3.3
作者：
Kerr, B. J.;Ziemer, C. J.;Parkin, T. B.
通讯作者：
Parkin, T. B.