Chlamydia and HPV induce centrosome amplification in the host cell through additive mechanisms.
Chlamydia and HPV induce centrosome amplification in the host cell through additive mechanisms.
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DOI：
10.1111/cmi.13397
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发表时间：
2021-12
影响因子：
3.4
通讯作者：
Sütterlin C
中科院分区：
文献类型：
--
作者：
Wang K;Muñoz KJ;Tan M;Sütterlin C
基于流行病学研究，沙眼衣原体被认为是人乳头瘤病毒（HPV）在宫颈癌发展中的辅助因素。这两种细胞内病原体已被独立报道诱导额外中心体的产生，或中心体扩增，这是癌细胞的标志。我们开发了一个细胞培养模型来系统地测量衣原体和HPV对同一宿主细胞中心体的单独和联合影响。我们发现沙眼衣原体比HPV在更大比例的细胞中引起中心体扩增，并且两种病原体对中心体的影响是加性的。此外，衣原体而非HPV诱导的中心体扩增与多核密切相关，并需要通过有丝分裂进行进展。我们的研究结果表明，沙眼原体和HPV通过不同的机制诱导中心体扩增，衣原体效应主要是由于细胞质分裂失败导致多核。我们的研究结果为沙眼原体作为HPV致癌的辅助因子提供了支持，并为两种感染因子如何合作促进癌症提供了机制见解。
Based on epidemiology studies, Chlamydia trachomatis has been proposed as a co-factor for human papillomavirus (HPV) in the development of cervical cancer. These two intracellular pathogens have been independently reported to induce the production of extra centrosomes, or centrosome amplification, which is a hallmark of cancer cells. We developed a cell culture model to systematically measure the individual and combined effects of Chlamydia and HPV on the centrosome in the same host cell. We found that C. trachomatis caused centrosome amplification in a greater proportion of cells than HPV and that the effects of the two pathogens on the centrosome were additive. Furthermore, centrosome amplification induced by Chlamydia, but not by HPV, strongly correlated with multinucleation and required progression through mitosis. Our results suggest that C. trachomatis and HPV induce centrosome amplification through different mechanisms with the chlamydial effect being largely due to a failure in cytokinesis that also results in multinucleation. Our findings provide support for C. trachomatis as a co-factor for HPV in carcinogenesis and offer mechanistic insights into how two infectious agents may cooperate to promote cancer.
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影响因子：
9.2
作者：
Chan JY
通讯作者：
Chan JY
DOI：
10.1073/pnas.95.6.2950
发表时间：
1998-03-17
影响因子：
11.1
作者：
Lingle, WL;Lutz, WH;Salisbury, JL
通讯作者：
Salisbury, JL
影响因子：
8
作者：
Duensing, A.;Liu, Y.;Duensing, S.
通讯作者：
Duensing, S.
影响因子：
56.9
作者：
Bunz, F;Dutriaux, A;Vogelstein, B
通讯作者：
Vogelstein, B
影响因子：
3.7
作者：
Buckner LR;Amedee AM;Albritton HL;Kozlowski PA;Lacour N;McGowin CL;Schust DJ;Quayle AJ
通讯作者：
Quayle AJ