The complete genome sequences of poxviruses isolated from a penguin and a pigeon in South Africa and comparison to other sequenced avipoxviruses.
The complete genome sequences of poxviruses isolated from a penguin and a pigeon in South Africa and comparison to other sequenced avipoxviruses.
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DOI：
10.1186/1471-2164-15-463
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发表时间：
2014-06-12
期刊：
影响因子：
4.4
通讯作者：
Williamson AL
中科院分区：
文献类型：
--
作者：
Offerman K;Carulei O;van der Walt AP;Douglass N;Williamson AL
来自南非的两种新型禽痘病毒已被测序，一种来自野鸽（Columba livia）（FeP 2），另一种来自非洲企鹅（Spheniscus demersus）（PEPV）。我们提出了一个专门设计的生物信息学管道，用于分析禽痘病毒的下一代序列数据，并比较迄今为止测序的不同禽痘病毒，特别强调它们的进化和基因内容。FeP 2（282 kbp）和PEPV（306 kbp）基因组分别编码271和284个开放阅读框，它们之间的亲缘关系（94.4%）比与鸡痘病毒（FWPV）（分别为85.3%和84.0%）或金丝雀痘病毒（CNPV）（分别为62.0%和63.4%）更近。总的来说，FeP 2，PEPV和FWPV具有同线基因排列;然而，在它们的基因组中存在重大差异。FeP 2和FWPV样禽痘病毒之间最显著的差异是FeP 2基因组中心区域约16 kbp的大缺失，缺失了一个α-趋化因子样基因、两个天花病毒B22 R直向同源物、一个N1 R/p28样基因和一个V型IG结构域家族基因。FeP 2和PEPV都编码牛痘病毒C7 L和白细胞介素10的直向同源物。PEPV含有77个氨基酸长的泛素直向同源物，与人泛素具有97%的氨基酸同一性。FeP 2和PEPV的基因组序列极大地增加了禽痘病毒属可用的有限基因组信息库。在FeP 2和PEPV与现有序列FWPV和CNPV的比较中，我们已经建立了对非洲禽痘病毒进化的见解。我们的数据支持这些南非禽痘病毒从一个共同的祖先病毒FWPV和CNPV的独立演变。
Two novel avipoxviruses from South Africa have been sequenced, one from a Feral Pigeon (Columba livia) (FeP2) and the other from an African penguin (Spheniscus demersus) (PEPV). We present a purpose-designed bioinformatics pipeline for analysis of next generation sequence data of avian poxviruses and compare the different avipoxviruses sequenced to date with specific emphasis on their evolution and gene content. The FeP2 (282 kbp) and PEPV (306 kbp) genomes encode 271 and 284 open reading frames respectively and are more closely related to one another (94.4%) than to either fowlpox virus (FWPV) (85.3% and 84.0% respectively) or Canarypox virus (CNPV) (62.0% and 63.4% respectively). Overall, FeP2, PEPV and FWPV have syntenic gene arrangements; however, major differences exist throughout their genomes. The most striking difference between FeP2 and the FWPV-like avipoxviruses is a large deletion of ~16 kbp from the central region of the genome of FeP2 deleting a cc-chemokine-like gene, two Variola virus B22R orthologues, an N1R/p28-like gene and a V-type Ig domain family gene. FeP2 and PEPV both encode orthologues of vaccinia virus C7L and Interleukin 10. PEPV contains a 77 amino acid long orthologue of Ubiquitin sharing 97% amino acid identity to human ubiquitin. The genome sequences of FeP2 and PEPV have greatly added to the limited repository of genomic information available for the Avipoxvirus genus. In the comparison of FeP2 and PEPV to existing sequences, FWPV and CNPV, we have established insights into African avipoxvirus evolution. Our data supports the independent evolution of these South African avipoxviruses from a common ancestral virus to FWPV and CNPV.
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DOI：
10.3390/v2091933
发表时间：
2010-09
期刊：
Viruses
影响因子：
--
作者：
Hendrickson RC;Wang C;Hatcher EL;Lefkowitz EJ
通讯作者：
Lefkowitz EJ
影响因子：
3.7
作者：
GOEBEL, SJ;JOHNSON, GP;PAOLETTI, E
通讯作者：
PAOLETTI, E
影响因子：
5.8
作者：
Brodie, R;Roper, RL;Upton, C
通讯作者：
Upton, C
影响因子：
14.9
作者：
Benson, G
通讯作者：
Benson, G
影响因子：
3.7
作者：
Darling AE;Mau B;Perna NT
通讯作者：
Perna NT