GWA analysis for milk production traits in dairy sheep and genetic support for a QTN influencing milk protein percentage in the LALBA gene.
GWA analysis for milk production traits in dairy sheep and genetic support for a QTN influencing milk protein percentage in the LALBA gene.
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GWA分析乳制绵羊中的牛奶生产特征和对QTN影响LALBA基因中牛奶蛋白百分比的遗传支持。
DOI：
10.1371/journal.pone.0047782
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Arranz JJ
中科院分区：
文献类型：
--
作者：
García-Gámez E;Gutiérrez-Gil B;Sahana G;Sánchez JP;Bayón Y;Arranz JJ
在这项研究中，我们使用Illumina OvineSNP 50 BeadChip进行全基因组关联（GWA）分析奶牛产奶性状通过分析西班牙Churra羊的商业人口。所研究的群体包括属于16个半同胞家庭的总共1，681只Churra母羊，其具有产奶量（MY）、乳蛋白和脂肪产量（PY和FY）以及乳蛋白和脂肪含量（PP和FP）的可用记录。最显着的协会确定达到实验意义的PP和FP和位于3号染色体（OAR 3）。这些结果证实了先前报道的影响PP的QTL的群体水平分离，并表明该QTL对FP具有显着的多效性效应。在其他14个染色体区域的染色体显著性水平上检测到进一步的关联。显示最高显著关联的OAR 3上的标记位于α-乳白蛋白（LALBA）基因的第三内含子，其是该关联的功能和位置候选者。测序这个基因在16个Churra公羊的研究资源人口确定了额外的多态性。在所鉴定的31个多态性中，有一个位于编码基因序列内（LALBA_g.242T>C），并被预测会导致蛋白质中的氨基酸变化（Val 27 Ala）。不同的方法，包括GWA分析，连锁和连锁不平衡研究和一致性测试的QTL分离状态的公畜，被用来评估的作用，这个突变作为一个假定的QTN的遗传效应检测OAR 3。我们的研究结果强烈支持多态性LALBA_g.242T>C作为所研究的影响PP和FP的OAR 3 QTL的最可能的因果突变，尽管我们不能排除该SNP与真正的因果多态性处于完全连锁不平衡的可能性。
In this study, we used the Illumina OvineSNP50 BeadChip to conduct a genome-wide association (GWA) analysis for milk production traits in dairy sheep by analyzing a commercial population of Spanish Churra sheep. The studied population consisted of a total of 1,681 Churra ewes belonging to 16 half-sib families with available records for milk yield (MY), milk protein and fat yields (PY and FY) and milk protein and fat contents (PP and FP). The most significant association identified reached experiment-wise significance for PP and FP and was located on chromosome 3 (OAR3). These results confirm the population-level segregation of a previously reported QTL affecting PP and suggest that this QTL has a significant pleiotropic effect on FP. Further associations were detected at the chromosome-wise significance level on 14 other chromosomal regions. The marker on OAR3 showing the highest significant association was located at the third intron of the alpha-lactalbumin (LALBA) gene, which is a functional and positional candidate underlying this association. Sequencing this gene in the 16 Churra rams of the studied resource population identified additional polymorphisms. One out of the 31 polymorphisms identified was located within the coding gene sequence (LALBA_g.242T>C) and was predicted to cause an amino acid change in the protein (Val27Ala). Different approaches, including GWA analysis, a combined linkage and linkage disequilibrium study and a concordance test with the QTL segregating status of the sires, were utilized to assess the role of this mutation as a putative QTN for the genetic effects detected on OAR3. Our results strongly support the polymorphism LALBA_g.242T>C as the most likely causal mutation of the studied OAR3 QTL affecting PP and FP, although we cannot rule out the possibility that this SNP is in perfect linkage disequilibrium with the true causal polymorphism.
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影响因子：
2.1
作者：
Diez-Tascón, C;Bayón, Y;San Primitivo, F
通讯作者：
San Primitivo, F
影响因子：
1.2
作者：
Gonda, Yuko;Sakurai, Hitoshi;Nakajima, Kazunori
通讯作者：
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影响因子：
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作者：
Legarra, Andres;Fernando, Rohan L.
通讯作者：
Fernando, Rohan L.
影响因子：
3.5
作者：
Gutierrez-Gil, B.;El-Zarei, M. F.;Arranz, J. J.
通讯作者：
Arranz, J. J.
影响因子：
3.5
作者：
Boston, WS;Bleck, GT;Miller, DJ
通讯作者：
Miller, DJ