Increased myocyte content and mechanical function within a tissue-engineered myocardial patch following implantation.
Increased myocyte content and mechanical function within a tissue-engineered myocardial patch following implantation.
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DOI：
10.1089/ten.tea.2008.0430
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发表时间：
2009-08
影响因子：
--
通讯作者：
Gaudette GR
中科院分区：
文献类型：
--
作者：
Kelly DJ;Rosen AB;Schuldt AJ;Kochupura PV;Doronin SV;Potapova IA;Azeloglu EU;Badylak SF;Brink PR;Cohen IS;Gaudette GR
在过去的几年里，涉及干细胞植入、化学因子和支架的研究已经证明了增强哺乳动物心脏天然再生能力的能力。由细胞外基质（ECM）组成的支架已被用于修复心肌缺损。这些支架被肌细胞填充，并提供区域收缩功能，但肌细胞数量的量化尚未进行。本研究的目的是量化ECM生物支架内的肌细胞含量，并将细胞群与区域机械功能随时间的变化联系起来。从猪膀胱中提取的异种ECM支架植入全层、手术诱导的狗右心室无壁缺损模型。植入后0、2和8周，测定每个贴片区域的区域功能和心肌细胞含量。0 ~ 2周区域功能无明显提高。然而，在8周时，局部脑卒中功增加到3.7±0.7%，收缩率增加到4.4±1.2%。在此期间，肌细胞含量也显著增加，产生了区域功能和肌细胞含量之间的线性关系。综上所述，随着时间的推移，ECM作为心肌贴片可以增加局部功能和心肌细胞含量。膜片区产生的机械功能与局部组织肌细胞的数量相关。
During the past few years, studies involving the implantation of stem cells, chemical factors, and scaffolds have demonstrated the ability to augment the mammalian heart’s native regenerative capacity. Scaffolds comprised of extracellular matrix (ECM) have been used to repair myocardial defects. These scaffolds become populated with myocytes and provide regional contractile function, but quantification of the myocyte population has not yet been conducted. The purpose of this study was to quantitate the myocyte content within the ECM bioscaffold and to correlate this cell population with the regional mechanical function over time. Xenogenic ECM scaffolds derived from porcine urinary bladder were implanted into a full-thickness, surgically induced, right ventricular-free wall defect in a dog model. Zero, 2, and 8 weeks following implantation, regional function and myocyte content were determined in each patch region. Regional function did not significantly increase from 0 to 2 weeks. At 8 weeks, however, regional stroke work increased to 3.7 ± 0.7% and systolic contraction increased to 4.4 ± 1.2%. The myocyte content also significantly increased during that period generating a linear relationship between regional function and myocyte content. In conclusion, ECM used as a myocardial patch increases both the regional function and the myocyte content over time. The mechanical function generated in the patch region is correlated with the quantity of local tissue myocytes.
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影响因子：
56.9
作者：
Poss, KD;Wilson, LG;Keating, MT
通讯作者：
Keating, MT
影响因子：
37.8
作者：
Ozawa, T;Mickle, DAG;Li, RK
通讯作者：
Li, RK
影响因子：
20.1
作者：
FENELEY, MP;ELBEERY, JR;RANKIN, JS
通讯作者：
RANKIN, JS
影响因子：
2.2
作者：
Kelly, Damon J.;Azeloglu, Evren U.;Gaudette, Glenn R.
通讯作者：
Gaudette, Glenn R.
影响因子：
6
作者：
Ozawa, T;Mickle, DAG;Li, RK
通讯作者：
Li, RK