Differential actions of isoflurane and ketamine-based anaesthetics on cochlear function in the mouse.
Differential actions of isoflurane and ketamine-based anaesthetics on cochlear function in the mouse.
复制标题
DOI：
10.1016/j.heares.2012.08.010
复制
发表时间：
2012-10
期刊：
影响因子：
2.8
通讯作者：
Housley, Gary D.
中科院分区：
文献类型：
--
作者：
Cederholm, Jennie M. E.;Froud, Kristina E.;Wong, Ann C. Y.;Ko, Myungseo;Ryan, Allen F.;Housley, Gary D.
异氟醚是一种挥发性吸入麻醉剂，广泛用于动物研究，在听力研究中具有特殊用途。与清醒状态相比，异氟醚已被证明能减弱听觉敏感性，但关于异氟醚与其他麻醉剂在毛细胞转导和听觉神经传递方面的比较，我们所知甚少。本研究在小鼠C57Bl/6J和C129/SvEv品系中进行，以确定相对于氯胺酮麻醉，异氟醚麻醉是否影响听力功能。使用听觉脑干反应（ABR）和畸变产物耳声发射（DPOAE）评估耳蜗功能和中枢听觉传递，比较异氟醚与氯胺酮/噻嗪/乙酰丙嗪麻醉的阈值和输入/输出功能随时间的变化。在异氟醚麻醉下，听觉最敏感区域（16 kHz）的ABR阈值最初较高。这种听觉敏感性的降低在1小时的研究期间恶化，并且在宽带点击刺激下也变得明显。氯胺酮麻醉提供稳定的ABR阈值。尽管两种麻醉药的生长功能随时间不变，但异氟醚麻醉下的斜率明显减少。两种麻醉剂的立方（2f1-f2） DPOAE阈值和生长功能最初相似。60分钟后，两组的DPOAE阈值均升高，但氯胺酮麻醉的效果更明显。随着时间的推移，异氟醚介导的ABR阈值升高可归因于对耳蜗神经激活的作用，与K/X/ a相比，P1-N1输入/输出函数的右移是显而易见的。尽管外毛细胞介导的DPOAE输入/输出功能发生右移，但基于氯胺酮的麻醉产生稳定的ABR阈值并随时间增加。
Isoflurane is a volatile inhaled anaesthetic widely used in animal research, with particular utility for hearing research. Isoflurane has been shown to blunt hearing sensitivity compared with the awake state, but little is known about how isoflurane compares with other anaesthetics with regard to hair cell transduction and auditory neurotransmission. The current study was undertaken in C57Bl/6J and C129/SvEv strains of mice to determine whether isoflurane anaesthesia affects hearing function relative to ketamine-based anaesthesia. Cochlear function and central auditory transmission were assessed using auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE), comparing thresholds and input/output functions over time, for isoflurane vs. ketamine/xylazine/acepromazine anaesthesia. ABR thresholds at the most sensitive region of hearing (16 kHz) were initially higher under isoflurane anaesthesia. This reduced hearing sensitivity worsened over the 1 h study period, and also became evident with broadband click stimulus. Ketamine anaesthesia provided stable ABR thresholds. Although the growth functions were unchanged over time for both anaesthetics, the slopes under isoflurane anaesthesia were significantly less. Cubic (2f1–f2) DPOAE thresholds and growth functions were initially similar for both anaesthetics. After 60 min, DPOAE thresholds increased for both groups, but this effect was significantly greater with ketamine anaesthesia. The isoflurane-mediated increase in ABR thresholds over time is attributable to action on cochlear nerve activation, evident as a right-shift in the P1-N1 input/output function compared to K/X/A. The ketamine-based anaesthetic produced stable ABR thresholds and gain over time, despite a right-shift in the outer hair cell – mediated DPOAE input/output function.
登录
查看更多内容
影响因子：
7.3
作者：
Chau PL
通讯作者：
Chau PL
DOI：
10.1523/jneurosci.3389-10.2011
发表时间：
2011-01-19
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Liberman LD;Wang H;Liberman MC
通讯作者：
Liberman MC
影响因子：
6.7
作者：
Kemp, DT
通讯作者：
Kemp, DT
影响因子：
8.8
作者：
DILGER, JP;VIDAL, AM;LIU, Y
通讯作者：
LIU, Y
影响因子：
8.8
作者：
de Sousa, SLM;Dickinson, R;Franks, NP
通讯作者：
Franks, NP