The Scattering of Gold Nanorods Combined with Differential Uptake, Paving a New Detection Method for Macrophage Subtypes Using Flow Cytometery.
The Scattering of Gold Nanorods Combined with Differential Uptake, Paving a New Detection Method for Macrophage Subtypes Using Flow Cytometery.
复制标题
DOI：
10.1021/acs.nanolett.0c03525
复制
发表时间：
2020-11-11
期刊：
影响因子：
10.8
通讯作者：
Fixler D
中科院分区：
文献类型：
--
作者：
Chakraborty R;Leshem-Lev D;Kornowski R;Fixler D
M1和M2巨噬细胞在体内和体外的鉴定策略将有助于预测个体的健康状况。在这里，我们介绍了一种解决这个问题的方法，它具有巨噬细胞的吞噬性质和金纳米棒（GNRs）的散射效应。内化的GNRs，与它们的摄入程度有关，在流式细胞术中的红色通道处引起明显的散射分布，推翻了细胞侧散射的贡献。这种内化完全由GNR的表面化学控制。PAH-GNRs显示出最大的摄入效力，其次是Cit-、PSS-和PEG-GNRs。值得注意的是，PAH-GNRs导致M1和M2细胞之间的摄取差异，M2细胞的摄取量是M1的三倍。这是第一次报告采用散射的未标记GNRs区分M1和M2细胞类型，使用流式细胞仪。
The strategy of identification for M1 and M2 macrophages both in vivo and in vitro would help to predict the health condition of the individual. Here, we introduced a solution to this problem with the advantage of both the phagocytic nature of macrophages and the scattering effect of gold nanorods (GNRs). The internalized GNRs, relating to their extent of intake, caused a conspicuous scattering profile at the red channel in flow cytometry, overruling the contribution of the cellular side scatters. This internalization is solely governed by the surface chemistry of GNRs. The PAH-GNRs showed maximum intake potency followed by Cit-, PSS-, and PEG-GNRs. On a substantial note, PAH-GNRs lead to differential uptake between M1 and M2 cells, with three times higher intake in M2 cells over M1. This is the first report of employing the scattering of unlabeled GNRs to discriminate M1 and M2 cell types using a flow cytometer.
登录
查看更多内容
影响因子：
4.1
作者：
Ankri R;Chakraborty R;Motiei M;Fixler D
通讯作者：
Fixler D
DOI：
10.1038/nri3520
发表时间：
2013-10
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
8
作者：
Ankri R;Melzer S;Tarnok A;Fixler D
通讯作者：
Fixler D
影响因子：
2.8
作者：
Chalcraborty, Ruchira;Ankri, Rinat;Fixler, Dror
通讯作者：
Fixler, Dror
影响因子：
3.3
作者：
Jain, PK;Lee, KS;El-Sayed, MA
通讯作者：
El-Sayed, MA