Cardiac extracellular proteome profiling and membrane topology analysis using glycoproteomics.
Cardiac extracellular proteome profiling and membrane topology analysis using glycoproteomics.
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DOI：
10.1002/prca.201400009
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发表时间：
2014-08
影响因子：
2
通讯作者：
Lindsey, Merry L.
中科院分区：
文献类型：
--
作者：
Tian, Yuan;Koganti, Tejaswi;Yao, Zhihao;Cannon, Presley;Shah, Punit;Pietrovito, Laura;Modesti, Alessandra;Aiyetan, Paul;DeLeon-Pennell, Kristine;Ma, Yonggang;Halade, Ganesh V.;Hicks, Chindo;Zhang, Hui;Lindsey, Merry L.
关键词：
细胞外蛋白很容易获得，它提供了具有高诊断和治疗潜力的分子靶标的亚蛋白质组。人们已经努力对心脏细胞外矩阵进行编目并分析已识别蛋白质的拓扑结构以设计治疗靶点。尽管已经开发了许多生物信息学工具来预测蛋白质拓扑结构，但仅对极小部分膜蛋白的拓扑结构进行了实验验证。本研究的目的是利用糖蛋白组学和质谱方法鉴定梗死左心室胞外基质中的糖蛋白，并为拓扑测定提供实验证据。使用固相提取糖肽，对来自野生型小鼠的 MI 后第 7 天样本的八个生物重复进行糖蛋白组学分析，然后通过质谱鉴定 N 连接糖基化位点以进行拓扑评估。我们鉴定了数百种糖蛋白，并且鉴定的 N-糖基化位点提供了有关梗塞环境中膜蛋白正确拓扑的新信息。我们的数据为未来左室梗塞细胞外基质的研究奠定了基础，这可能有助于药物靶点和生物标志物的发现。
Extracellular proteins are easily accessible, which presents a sub-proteome of molecular targets that have high diagnostic and therapeutic potential. Efforts have been made to catalogue the cardiac extracellular matridome and analyze the topology of identified proteins for the design of therapeutic targets. Although many bioinformatics tools have been developed to predict protein topology, topology has been experimentally validated for only a very small portion of membrane proteins. The aim of this study was to use a glycoproteomics and mass spectrometry approach to identify glycoproteins in the extracellular matridome of the infarcted LV and provide experimental evidence for topological determination. Glycoproteomics analysis was performed on eight biological replicates of day 7 post-MI samples from wild type mice using solid-phase extraction of glycopeptides, followed by mass spectrometric identification of N-linked glycosylation sites for topology assessment. We identified hundreds of glycoproteins and the identified N-glycosylation sites provide novel information on the correct topology for membrane proteins present in the infarct setting. Our data provides the foundation for future studies of the LV infarct extracellular matridome, which may facilitate the discovery of drug targets and biomarkers.
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影响因子：
4.4
作者：
Tian, Yuan;Kelly-Spratt, Karen S.;Kemp, Christopher J.;Zhang, Hui
通讯作者：
Zhang, Hui
DOI：
10.1074/mcp.m110.001693
发表时间：
2010-09
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Didangelos A;Yin X;Mandal K;Baumert M;Jahangiri M;Mayr M
通讯作者：
Mayr M
影响因子：
14.9
作者：
Gnad F;Gunawardena J;Mann M
通讯作者：
Mann M
影响因子：
13.5
作者：
Halade, Ganesh V.;Jin, Yu-Fang;Lindsey, Merry L.
通讯作者：
Lindsey, Merry L.
影响因子：
4.4
作者：
Swaney, Danielle L.;Wenger, Craig D.;Coon, Joshua J.
通讯作者：
Coon, Joshua J.