Development and characterization of an aerobic bacterial consortium for autotrophic biodegradation of thiocyanate
Development and characterization of an aerobic bacterial consortium for autotrophic biodegradation of thiocyanate
复制标题
用于硫氰酸盐自养生物降解的需氧细菌群落的开发和表征
DOI：
10.1016/j.cej.2020.125461
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发表时间：
2020-10
影响因子：
15.1
通讯作者：
Jianfei Luo
中科院分区：
文献类型：
--
作者：
Xinyi Wang;Liangting Liu;Weitie Lin;Jianfei Luo
在SCN−生物修复过程中，使用微生物菌群似乎比使用单一细菌具有更高的去除率和更高的去除效率，特别是在连续生物反应器中。然而，对这一现象的解释却很少。在这里，我们获得了一个好氧自养细菌财团从河流沉积物经过一个月的富集。它能够耐受和降解高达70 mM的SCN−在分批培养，并有1.4 mmol L−1h− 1在连续生物膜反应器的最大去除率。高通量测序和宏基因组学分析表明，硫杆菌属（Thiobacillus）是该菌群中的优势菌，分别占总菌群的43%和58%。沿着仅在硫杆菌中鉴定出SCN酶编码基因，该细菌群被认为是负责SCN−去除的主要贡献者。从细菌聚生体中分离出七种细菌菌株;只有属于硫杆菌属的分离物被确定能够降解SCN−。特别是在其他硫化物氧化细菌（噬氢菌）存在下，硫杆菌菌株可以获得比单独使用更高的去除效率。根据基于基因组的分析，提出了SCN−降解自养生物和硫化物氧化异养生物之间的交叉喂养模式，这使得个体之间的合作比单一细菌群体更有效地去除SCN−。这项研究为SCN-降解提供了新的见解，并对为什么联合体更适合SCN-去除给出了潜在的解释。
During the SCN−bioremediation, the use of microbial consortia seems to have higher removal rate and steadier removal efficiency than the use of single bacterium, especially in the continuous bioreactor. However, little explanation has been given towards this phenomenon. Here we obtained an aerobic autotrophic bacterial consortium from river sediment after one month of enrichment. It was able to tolerate and degrade up to 70 mM SCN−in batch culture and had a maximum removal rate of 1.4 mmol L−1h−1in a continuous biofilm reactor. High-throughput sequencing and metagenomic analysis revealed that the genusThiobacilluswas the predominant member in the bacterial consortium, which accounted for 43% and 58% of the total communities, respectively. Along with the identification of SCNase encoding genes only inThiobacillus, this bacterial group was recognized as the main contributor responsible for the SCN−removal. Seven bacterial strains were isolated from the bacterial consortium; only the isolate belonging toThiobacilluswas determined to be able to degrade SCN−. In particular, in the presence of other sulfide-oxidizing bacterium (Hydrogenophaga), theThiobacillusstrain could achieve higher removal efficiency than it alone. According to the genome-based analysis, a cross-feeding pattern between the SCN−-degrading autotrophs and sulfide-oxidizing heterotrophs was proposed, which makes the cooperation between individuals more effective than the single bacterial group for the SCN−removal. This study provides a new insight into the SCN−degradation and gives a potential explanation as to why consortium would be more suitable for the SCN−removal.
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DOI：
10.1099/00207713-52-2-657
发表时间：
2002-03-01
影响因子：
2.8
作者：
Sorokin, DY;Tourova, TP;Kuenen, JG
通讯作者：
Kuenen, JG
DOI：
10.1099/ijs.0.012880-0
发表时间：
2010-02
影响因子：
2.8
作者：
D. Sorokin;O. Kovaleva;T. Tourova;G. Muyzer
通讯作者：
D. Sorokin;O. Kovaleva;T. Tourova;G. Muyzer
影响因子：
12.8
作者：
Se-jeong Kim;Y. Katayama
通讯作者：
Se-jeong Kim;Y. Katayama
DOI：
10.1039/c4cp05975a
发表时间：
2015-05
期刊：
Physical chemistry chemical physics : PCCP
影响因子：
--
作者：
Paolo Piazzetta;T. Marino;N. Russo
通讯作者：
Paolo Piazzetta;T. Marino;N. Russo
影响因子：
12.8
作者：
M. Watts;L. P. Spurr;K. Lê Cao;R. Wick;J. Banfield;J. Moreau
通讯作者：
M. Watts;L. P. Spurr;K. Lê Cao;R. Wick;J. Banfield;J. Moreau