Association of chemerin mRNA expression in human epicardial adipose tissue with coronary atherosclerosis.
Association of chemerin mRNA expression in human epicardial adipose tissue with coronary atherosclerosis.
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DOI：
10.1186/1475-2840-10-87
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发表时间：
2011-10-07
影响因子：
9.3
通讯作者：
Tao H
中科院分区：
文献类型：
--
作者：
Gao X;Mi S;Zhang F;Gong F;Lai Y;Gao F;Zhang X;Wang L;Tao H
越来越多的证据表明，心外膜脂肪组织（EAT）可能通过产生多种炎性脂肪因子在冠状动脉疾病（CAD）的发病和发展中起关键作用。Chemerin是一种新的脂肪细胞因子，参与调节免疫反应和糖脂代谢。鉴于这些特性，chemerin可能提供肥胖，炎症和动脉粥样硬化之间的有趣联系。在这项研究中，我们试图确定中国汉族患者EAT中chemerin表达与冠状动脉粥样硬化严重程度的关系。从接受择期心脏手术的CAD（n = 37）和NCAD（n = 16）患者中获得血清和脂肪组织活检（心外膜和胸部皮下）。采用Gensini评分评估CAD的严重程度。ELISA法测定血清chemerin、脂联素和胰岛素水平。免疫组化法检测脂肪组织中Chemerin蛋白的表达。RT-PCR检测脂肪组织中chemerin、chemR 23、adiponectin和TNF-α的mRNA水平。结果显示，冠心病组EAT中chemerin和TNF-α mRNA水平显著高于非冠心病组，而脂联素mRNA水平显著低于非冠心病组。CAD组EAT中chemerin mRNA和蛋白表达水平显著高于配对皮下脂肪组织（SAT），而NCAD组EAT中chemerin mRNA和蛋白表达水平无显著差异。EAT中Chemerin mRNA表达与Gensini评分呈正相关（r = 0.365，P < 0.05），校正年龄、性别、BMI和腰围后，EAT中Chemerin mRNA表达与Gensini评分的相关性仍有统计学意义（r = 0.357，P < 0.05）。EAT中Chemerin mRNA表达与BMI（r = 0.305，P < 0.05）、腰围（r = 0.384，P < 0.01）、空腹血糖（r = 0.334，P < 0.05）呈正相关，与脂联素mRNA表达呈负相关（r =-0.322，P < 0.05）。然而，两组之间的血清chemerin或脂联素水平无显著差异。同样，血清chemerin和血清脂联素与Gensini评分均无相关性（P > 0.05）。中国汉族冠心病患者EAT中chemerin mRNA和蛋白的表达均显著增高。此外，冠状动脉粥样硬化的严重程度与EAT中chemerin mRNA的水平呈正相关，而不是其循环水平。
Growing evidence suggests that epicardial adipose tissue (EAT) may play a key role in the pathogenesis and development of coronary artery disease (CAD) by producing several inflammatory adipokines. Chemerin, a novel adipokine, has been reported to be involved in regulating immune responses and glucolipid metabolism. Given these properties, chemerin may provide an interesting link between obesity, inflammation and atherosclerosis. In this study, we sought to determine the relationship of chemerin expression in EAT and the severity of coronary atherosclerosis in Han Chinese patients. Serums and adipose tissue biopsies (epicardial and thoracic subcutaneous) were obtained from CAD (n = 37) and NCAD (n = 16) patients undergoing elective cardiac surgery. Gensini score was used to assess the severity of CAD. Serum levels of chemerin, adiponectin and insulin were measured by ELISA. Chemerin protein expression in adipose tissue was detected by immunohistochemistry. The mRNA levels of chemerin, chemR23, adiponectin and TNF-alpha in adipose tissue were detected by RT-PCR. We found that EAT of CAD group showed significantly higher levels of chemerin and TNF-alpha mRNA, and significantly lower level of adiponectin mRNA than that of NCAD patients. In CAD group, significantly higher levels of chemerin mRNA and protein were observed in EAT than in paired subcutaneous adipose tissue (SAT), whereas such significant difference was not found in NCAD group. Chemerin mRNA expression in EAT was positively correlated with Gensini score (r = 0.365, P < 0.05), moreover, this correlation remained statistically significant (r = 0.357, P < 0.05) after adjusting for age, gender, BMI and waist circumference. Chemerin mRNA expression in EAT was also positively correlated with BMI (r = 0.305, P < 0.05), waist circumference (r = 0.384, P < 0.01), fasting blood glucose (r = 0.334, P < 0.05) and negatively correlated with adiponectin mRNA expression in EAT (r = -0.322, P < 0.05). However, there were no significant differences in the serum levels of chemerin or adiponectin between the two groups. Likewise, neither serum chemerin nor serum adiponectin was associated with Gensini score (P > 0.05). The expressions of chemerin mRNA and protein are significantly higher in EAT from patients with CAD in Han Chinese patients. Furthermore, the severity of coronary atherosclerosis is positive correlated with the level of chemerin mRNA in EAT rather than its circulating level.
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DOI：
10.1152/ajpendo.90511.2008
发表时间：
2009-06
期刊：
American journal of physiology. Endocrinology and metabolism
影响因子：
--
作者：
Madani R;Karastergiou K;Ogston NC;Miheisi N;Bhome R;Haloob N;Tan GD;Karpe F;Malone-Lee J;Hashemi M;Jahangiri M;Mohamed-Ali V
通讯作者：
Mohamed-Ali V
影响因子：
37.8
作者：
Mazurek, T;Zhang, LF;Shi, Y
通讯作者：
Shi, Y
影响因子：
9.3
作者：
Baker, Adam R.;da Silva, Nancy F.;Quinn, David W.;Harte, Alison L.;Pagano, Domenico;Bonser, Robert S.;Kumar, Sudhesh;McTernan, Philip G.
通讯作者：
McTernan, Philip G.
影响因子：
5.8
作者：
Baker, A. R.;Harte, A. L.;McTernan, P. G.
通讯作者：
McTernan, P. G.
DOI：
10.1016/j.carpath.2010.07.005
发表时间：
2011-09-01
期刊：
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology
影响因子：
--
作者：
Bambace, Clara;Telesca, Mariassunta;Zamboni, Mauro
通讯作者：
Zamboni, Mauro