Regulation of WOX11 Expression Represents the Difference Between Direct and Indirect Shoot Regeneration.
Regulation of WOX11 Expression Represents the Difference Between Direct and Indirect Shoot Regeneration.
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WOX11 表达的调节代表了直接和间接芽再生之间的差异
DOI：
10.3389/fpls.2022.850726
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发表时间：
2022
影响因子：
5.6
通讯作者：
Cheng ZJ
中科院分区：
文献类型：
--
作者：
Liu JH;Dong WC;Fei FF;Li XT;Zhang XH;Zhou Y;Zhang XS;Sang YL;Cheng ZJ
高等植物体细胞具有通过重建顶端分生组织再生新个体的显著能力。茎部分生组织的重组是植物生物技术应用的重要过程。在离体培养条件下，茎的分生组织可以直接或间接形成，这取决于是否存在愈伤组织作为中间状态。然而，两种再生类型之间的调控机制差异尚不清楚。在本研究中，我们建立了一个直接从侧根原基（LRP）和间接从下胚轴衍生的愈伤组织同时再生枝条的双向系统。基于该系统的研究结果表明，WOX11的表达调控在两个方面代表了两种再生类型的差异。首先，表达WOX11的创始细胞数量与再生类型密切相关。相对而言，较多的创始细胞产生愈伤组织并产生较大的分生组织，而较少的创始细胞产生LRP，产生较小的分生组织。其次，非cg DNA甲基化特异性调控了LRP中WOX11的转录，促进了直接茎再生，但对间接再生没有影响。这些结果为理解新生器官发生过程中细胞命运转变的调控机制提供了新的见解。
Somatic cells of higher plants possess the remarkable ability to regenerate new individuals via reestablishing apical meristems. Reconstitution of shoot meristem is the vital process and is required for application of plant biotechnology. Under in vitro culture condition, shoot meristem can be formed directly or indirectly, depending on the absence or presence of callus as the intermediate status. However, the difference of regulatory mechanisms between the two regeneration types remains unknown. In this study, we established a bi-directional system in which shoots regenerated directly from lateral root primordia (LRP) and indirectly from hypocotyl-derived callus simultaneously. The results based on this system revealed that regulation of WOX11 expression represents the difference between the two regeneration types in two aspects. Firstly, number of founder cells expressing WOX11 is tightly associated with regeneration types. Relatively more founder cells gave rise to callus and produce larger meristem, whereas less founder cells produce LRP that regenerate smaller meristem. Secondly, non-CG DNA methylation specifically regulated WOX11 transcription in LRP and promoted direct shoot regeneration, but had no influence on indirect regeneration. The results provide new insights for understanding the regulatory mechanisms of cell fate transition during de novo organogenesis.
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影响因子：
16.8
作者：
Stroud H;Do T;Du J;Zhong X;Feng S;Johnson L;Patel DJ;Jacobsen SE
通讯作者：
Jacobsen SE
影响因子：
9.2
作者：
Heisler, MG;Ohno, C;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
4.3
作者：
Che, Ping;Lall, Sonia;Howell, Stephen H.
通讯作者：
Howell, Stephen H.
影响因子：
11.8
作者：
Sugimoto, Kaoru;Jiao, Yuling;Meyerowitz, Elliot M.
通讯作者：
Meyerowitz, Elliot M.
DOI：
10.1242/dev.152132
发表时间：
2017-09-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Sheng L;Hu X;Du Y;Zhang G;Huang H;Scheres B;Xu L
通讯作者：
Xu L