Microsatellite loci in Japanese quail and cross-species amplification in chicken and guinea fowl.
Microsatellite loci in Japanese quail and cross-species amplification in chicken and guinea fowl.
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DOI：
10.1186/1297-9686-34-2-233
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发表时间：
2002-03
期刊：
影响因子：
--
通讯作者：
Ito S
中科院分区：
文献类型：
--
作者：
Kayang BB;Inoue-Murayama M;Hoshi T;Matsuo K;Takahashi H;Minezawa M;Mizutani M;Ito S
根据岐阜大学绘制日本鹌鹑基因组图谱的倡议，我们实验室对从野生鹌鹑起源群体中随机采样的 20 只不相关的鹌鹑种群进行了评估，共评估了我们实验室分离的 100 个日本鹌鹑微卫星标记。 98 个标记是多态性的，每个位点平均有 3.7 个等位基因，平均杂合度为 0.423。为了确定这些标记在雉科比较基因组作图中的效用，用鸡和珍珠鸡 DNA 测试了所有标记的跨物种扩增。在鸡的 42 个基因座和珍珠鸡的 20 个基因座中观察到大小与鹌鹑的直系同源基因座相似的扩增产物。在对来自各自种群的 20 只鸟类进行测试时，交叉反应标记中，鸡中 57.1% 和珍珠鸡中 55.0% 具有多态性。能够交叉扩增日本鹌鹑、鸡和珍珠鸡 DNA 的 15 个标记中有 5 个在这三个物种中都是多态性的。通过对鸡和珍珠鸡各 10 个基因座进行测序并与相应的鹌鹑序列进行比较，证实了直系同源基因座的扩增。报告的微卫星标记将作为鹌鹑遗传图谱和雉科比较图谱的有用资源库。
In line with the Gifu University's initiative to map the Japanese quail genome, a total of 100 Japanese quail microsatellite markers isolated in our laboratory were evaluated in a population of 20 unrelated quails randomly sampled from a colony of wild quail origin. Ninety-eight markers were polymorphic with an average of 3.7 alleles per locus and a mean heterozygosity of 0.423. To determine the utility of these markers for comparative genome mapping in Phasianidae, cross-species amplification of all the markers was tested with chicken and guinea fowl DNA. Amplification products similar in size to the orthologous loci in quail were observed in 42 loci in chicken and 20 loci in guinea fowl. Of the cross-reactive markers, 57.1% in chicken and 55.0% in guinea fowl were polymorphic when tested in 20 birds from their respective populations. Five of 15 markers that could cross-amplify Japanese quail, chicken, and guinea fowl DNA were polymorphic in all three species. Amplification of orthologous loci was confirmed by sequencing 10 loci each from chicken and guinea fowl and comparing with them the corresponding quail sequence. The microsatellite markers reported would serve as a useful resource base for genetic mapping in quail and comparative mapping in Phasianidae.
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影响因子：
2.4
作者：
Inoue-Murayama, M;Kayang, BB;Ito, S
通讯作者：
Ito, S
影响因子：
4.4
作者：
CRITTENDEN, LB;PROVENCHER, L;DODGSON, JB
通讯作者：
DODGSON, JB
影响因子：
4.4
作者：
BUMSTEAD, N;PALYGA, J
通讯作者：
PALYGA, J
影响因子：
7
作者：
Primmer, CR;Raudsepp, T;Ellegren, H
通讯作者：
Ellegren, H
影响因子：
2.4
作者：
deGortari, MJ;Freking, BA;Beattie, CW
通讯作者：
Beattie, CW