The novel correlation of carbonic anhydrase II and anion exchanger 1 in gills of the spotted green pufferfish, Tetraodon nigrovirids.
The novel correlation of carbonic anhydrase II and anion exchanger 1 in gills of the spotted green pufferfish, Tetraodon nigrovirids.
复制标题
斑点绿河豚（Tetraodon nigrovirids）鳃中碳酸酐酶 II 和阴离子交换剂 1 的新相关性。
DOI：
10.1002/jez.391
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Lee,TH
中科院分区：
文献类型：
--
作者：
Tang,CH;Lee,TH
研究了广盐性斑点绿色河豚鳃碳酸酐酶II（CAII）与阴离子交换蛋白1（AE 1）之间的关系。免疫印迹显示，AE 1只检测到在膜部分的鳃，而CAII可以探测到在膜和细胞质部分的鳃。CAII蛋白在膜组分中的丰度是盐度依赖性的。免疫学检测显示，低渗（淡水）组中鳃中的膜组分CAII蛋白是高渗（海水;35‰）组的3.9倍。相反，海水和淡水组之间的细胞质CAII的蛋白水平没有变化。免疫细胞化学染色结果表明，AE 1和CAII共定位于河豚鳃上皮Na+/K+-ATP酶免疫反应阳性细胞。CAII和AE 1之间的相互作用通过免疫共沉淀进一步鉴定，因为AE 1在CAII的免疫沉淀物中检测到，反之亦然。我们的研究结果表明，在河豚鳃CAII不仅表达在胞质溶胶中，以产生在Cl−内流过程中的AE 1运输的底物，但也通过AE 1与质膜。显然，AE 1与鳃Na+/K+-ATP酶-免疫反应细胞膜上的CAII相互作用对于其生理功能至关重要。据我们所知，这是第一个研究，以证明在鱼类鳃CAII和AE 1的相互作用。新的相关性提出了一个新的模型Cl−/HCO 3 −运输的鳃的硬骨鱼。Zool.307A：411-418，2007.© 2007 Wiley利斯公司
A novel relationship between branchial carbonic anhydrase II (CAII) and anion exchanger 1 (AE1) was investigated in the euryhaline spotted green pufferfish (Tetraodon nigroviridis). The immunoblots revealed that AE1 was only detected in the membrane fraction of gills while CAII can be probed both in the membrane and cytosol fractions of gills. CAII protein abundance in the membrane fraction is salinity dependent. Immunological detection of the membrane fraction CAII protein in gills showed 3.9‐fold higher in the hyposmotic (freshwater) group than the hyperosmotic (seawater;35‰) group. In contrast, there was no change in the protein level of cytosolic CAII between seawater and freshwater groups. The whole‐mount immunocytochemical staining demonstrated that both AE1 and CAII were colocalized to the Na+/K+‐ATPase‐immunoreactive cells in gill epithelium of the pufferfish. The interaction between CAII and AE1 was further identified by co‐immunoprecipitation because AE1 was detected in the immunoprecipitates of CAII and vice versa. Our results showed that in pufferfish gills CAII was not only expressed in the cytosol to produce the substrate for AE1 transport during Cl−influx but also associated with the plasma membrane via AE1. Obviously, it is essential for the physiological function of AE1 to interact with CAII in the membrane of gill Na+/K+‐ATPase‐immunoreactive cells. To our knowledge, this is the first study to demonstrate the interaction of branchial CAII and AE1 in fish. The novel correlation proposed a new model of Cl−/HCO3−transport in gills of the teleosts.J. Exp. Zool. 307A:411–418, 2007. © 2007 Wiley‐Liss, Inc.
登录
查看更多内容
β 嵌入细胞的顶端 Cl/HCO3 交换器。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
vanAdelsberg,JS;Edwards,JC;al-Awqati,Q
通讯作者：
al-Awqati,Q
广盐黄貂鱼 (Dasyatis sabina) 鳃中液泡质子-ATP 酶 B 亚基的免疫化学分析：盐度的影响以及与 Na( )/K( )-ATP 酶的关系。
影响因子：
2.8
作者：
P. Piermarini;David H. Evans
通讯作者：
David H. Evans
H.Abe：Comp.Biochem.Physiol。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--
温驯化对南极鱼氯细胞Na,K-ATP酶α亚基表达的影响。
DOI：
10.1002/ar.a.20203
发表时间：
2005
期刊：
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
影响因子：
--
作者：
P. Brauer;J. Sanmann;D. Petzel
通讯作者：
D. Petzel
DOI：
10.1016/j.cbpb.2004.04.005
发表时间：
2004-07-01
影响因子：
2.3
作者：
Lin, CH;Tsai, RS;Lee, TH
通讯作者：
Lee, TH