Monoclonal antibodies that bind the renal Na+/glucose symport system. 1. Identification.
Monoclonal antibodies that bind the renal Na+/glucose symport system. 1. Identification.
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结合肾钠/葡萄糖转运系统的单克隆抗体。
DOI：
10.1021/bi00392a030
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Lever,JE
中科院分区：
文献类型：
--
作者：
Wu,JS;Lever,JE
Department of Biochemistry and Molecular Biology，The University of Texas Medical School，Houston，Texas 77225 Received October 13，1986; Revised Mandarin pt Received May 12，1987摘要：根皮苷是一种特异性的高亲和力配体，通过Na+依赖性机制结合Na+/葡萄糖同向转运体的活性位点，但本身不跨膜转运。我们已经分离出一组单克隆抗体，影响高亲和力，Na+依赖性根皮苷结合猪肾刷状缘膜。用高度纯化的肾刷状缘膜或从猪肾近端小管来源的细胞系LLC-PK]纯化的顶端膜免疫小鼠后衍生抗体。抗体11 A3 D 6，一种IgG 2b，可重复地刺激Na+依赖性根皮苷结合，而抗体18 H10 B12，一种IgM，强烈抑制特异性结合。这些作用在孵育30分钟后达到最大，并在抗体浓度增加时表现出饱和。抗体不影响囊泡中Na+依赖性糖的摄取，但显着阻止了结合根皮苷的transportinflammation。抗体识别刷状缘膜的Western印迹分析确定的75-kDa抗原，和75-kDa的膜蛋白可以通过18 H10 B12免疫沉淀。这些性质，结合以下论文[Wu，J.- S. R.，和Lever，JE（1987）Biochemistry（在这期中的后续论文）]提供了令人信服的证据，证明这些抗体识别的75-kDa抗原是肾Na+/葡萄糖同向转运体的组分。
Department of Biochemistry and Molecular Biology, The University of Texas Medical School, Houston, Texas 77225 Received October 13, 1986; Revised Manuscript Received May 12, 1987 abstract: Phlorizin is a specific, high-affinity ligand that binds the active site of the Na+/glucose symporter by a Na+-dependent mechanism but is not itself transported across the membrane. We have isolated a panel of monoclonal antibodies that influence high-affinity, Na+-dependent phlorizin binding to pig renal brush border membranes. Antibodies were derived after immunization of mice eitherwith highly purified renal brush border membranes or with apical membranes purified from LLC-PK], a cell line of pig renal proximal tubule origin. Antibody 11A3D6, an IgG2b, reproducibly stimulated Na+-dependent phlorizin binding whereas antibody 18H10B12, an IgM, strongly inhibited specific binding. These effects were maximal after 30-min incubation and exhibited saturation atincreased antibody concentrations. Antibodies did not affect Na+-dependent sugar uptake in vesicles but significantly prevented transportinhibition by bound phlorizin. Antibodies recognized a 75-kDa antigen identified by Western blot analysis of brush border membranes, and a 75-kDa membrane protein could be immunoprecipitated by 18H10B12. These properties, taken together with results in the following paper [Wu, J.-S. R., & Lever, JE (1987) Biochemistry (following paper in this issue)], provide compelling evidence that the 75-kDa antigen recognized by these antibodies is a component of the renal Na+/glucose symporter.
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从刷状缘膜中分离钠依赖性 d-葡萄糖转运蛋白。
DOI：
10.1016/0005-2736(83)90144-x
发表时间：
1983
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
P. Malathi;H. Preiser
通讯作者：
H. Preiser
氨基试剂对小肠 Na /D-葡萄糖协同转运蛋白的化学修饰。
DOI：
10.1016/0005-2736(83)90039-1
发表时间：
1983
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
J. Weber;G. Semenza
通讯作者：
G. Semenza
使用单克隆抗体分离兔肠刷状缘膜的 Na+/D-葡萄糖转运蛋白（的亚基）
影响因子：
3.5
作者：
U. Schmidt;B. Eddy;C. Fraser;J. Craig Venter;G. Semenza
通讯作者：
G. Semenza
针对大肠杆菌 lac 载体蛋白的单克隆抗体。
影响因子：
2.9
作者：
N. Carrasco;P. Viitanen;D. Herzlinger;H. Kaback
通讯作者：
H. Kaback
肾刷状缘膜根皮苷结合蛋白的辐射灭活研究。
DOI：
10.1016/s0021-9258(18)33894-8
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Turner;E. Kempner
通讯作者：
E. Kempner