Saturation diving alters folate status and biomarkers of DNA damage and repair.
Saturation diving alters folate status and biomarkers of DNA damage and repair.
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DOI：
10.1371/journal.pone.0031058
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Smith SM
中科院分区：
文献类型：
--
作者：
Zwart SR;Jessup JM;Ji J;Smith SM
暴露于富氧环境会导致氧化损伤，增加体内铁储存，以及某些维生素（包括叶酸）状态的变化。评估这些环境中氧化损伤的类型并确定其与叶酸状态变化的关系对于确定营养需求和设计减轻这些影响的对策非常重要。研究了在氧分压增加的环境中进行饱和潜水的参与者对人类氧化应激的反应，这是NASA极端环境使命行动使命。六名参与者完成了13-D饱和潜水的栖息地19米以下的海洋表面附近的基拉戈，佛罗里达州。空腹血液样本收集前，两次期间，两次潜水后，并分析铁的状态，氧化损伤和维生素状态的生化标志物。潜水过程中机体铁储备和铁蛋白增加（P<0.001），红细胞叶酸（P<0.001）和超氧化物歧化酶活性下降（P<0.001）。叶酸状态与血清铁蛋白相关（Pearson r =-0.34，P<0.05）。 外周血单个核细胞多聚腺苷二磷酸核糖（poly（ADP-ribose））在潜水过程中升高，潜水结束时升高显著（P<0.001），γ-H2 AX在使命中无变化。总之，这些数据提供的证据表明，当身体铁储存在高氧环境中升高时，外周血单核细胞中发生DNA损伤修复反应，但双链DNA损伤没有。此外，叶酸状态在这种环境中迅速下降，这项研究提供的证据表明，当身体铁储存和DNA损伤修复反应升高时，叶酸的需求可能会更大。
Exposure to oxygen-rich environments can lead to oxidative damage, increased body iron stores, and changes in status of some vitamins, including folate. Assessing the type of oxidative damage in these environments and determining its relationships with changes in folate status are important for defining nutrient requirements and designing countermeasures to mitigate these effects. Responses of humans to oxidative stressors were examined in participants undergoing a saturation dive in an environment with increased partial pressure of oxygen, a NASA Extreme Environment Mission Operations mission. Six participants completed a 13-d saturation dive in a habitat 19 m below the ocean surface near Key Largo, FL. Fasting blood samples were collected before, twice during, and twice after the dive and analyzed for biochemical markers of iron status, oxidative damage, and vitamin status. Body iron stores and ferritin increased during the dive (P<0.001), with a concomitant decrease in RBC folate (P<0.001) and superoxide dismutase activity (P<0.001). Folate status was correlated with serum ferritin (Pearson r = −0.34, P<0.05). Peripheral blood mononuclear cell poly(ADP-ribose) increased during the dive and the increase was significant by the end of the dive (P<0.001); γ-H2AX did not change during the mission. Together, the data provide evidence that when body iron stores were elevated in a hyperoxic environment, a DNA damage repair response occurred in peripheral blood mononuclear cells, but double-stranded DNA damage did not. In addition, folate status decreases quickly in this environment, and this study provides evidence that folate requirements may be greater when body iron stores and DNA damage repair responses are elevated.
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影响因子：
3.5
作者：
Henning, BF;Tepel, M;Naurath, HJ
通讯作者：
Naurath, HJ
影响因子：
--
作者：
Krog, Jan;Tonnesen, Else K.;Hokland, Marianne E.
通讯作者：
Hokland, Marianne E.
DOI：
10.1158/1078-0432.ccr-10-0523
发表时间：
2010-09-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Redon CE;Nakamura AJ;Zhang YW;Ji JJ;Bonner WM;Kinders RJ;Parchment RE;Doroshow JH;Pommier Y
通讯作者：
Pommier Y
影响因子：
4.2
作者：
Smith, SM;Davis-Street, JE;Zwart, SR
通讯作者：
Zwart, SR
影响因子：
4.2
作者：
Ho, PI;Ortiz, D;Shea, TB
通讯作者：
Shea, TB