Puerariae lobatae Radix Alleviates Pre-Eclampsia by Remodeling Gut Microbiota and Protecting the Gut and Placental Barriers.
Puerariae lobatae Radix Alleviates Pre-Eclampsia by Remodeling Gut Microbiota and Protecting the Gut and Placental Barriers.
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葛根通过重塑肠道菌群并保护肠道和胎盘屏障来缓解先兆子痫
DOI：
10.3390/nu14235025
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发表时间：
2022-11-25
期刊：
影响因子：
5.9
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Huang L;Liu Z;Wu P;Yue X;Lian Z;He P;Liu Y;Zhou R;Zhao J
子痫前期（Pre-eclampsia，PE）是一种严重的妊娠并发症，肠道菌群失调是其重要原因之一。本研究旨在探讨PLR缓解PE的疗效及其机制。我们使用NG-硝基-L-精氨酸甲酯（L-NAME）诱导的PE小鼠模型来检查预防和治疗PLR补充的功效。结果表明，PLR干预减轻高血压和蛋白尿，增加胎儿和胎盘重量，并提高VEGF和PlGF的水平。此外，PLR通过激活Nrf 2/HO-1/NQO 1通路保护胎盘免受氧化应激，并通过增加肠屏障标志物（ZO-1，Occludin和Claudin-1）表达和减少脂多糖渗漏来减轻胎盘损伤。值得注意的是，预防性PLR施用纠正了PE小鼠中的肠道生态失调，如通过有益细菌（包括双歧杆菌属、布劳特氏菌属和Turicibacter）的丰度增加和积极相互作用所证明的。粪便微生物群移植证实肠道微生物群部分介导PLR对PE的有益作用。我们的研究结果表明，调节肠道微生物群是治疗PE的有效策略，并强调PLR可用作PE患者的肠道营养补充剂。
Pre-eclampsia (PE) is a serious pregnancy complication, and gut dysbiosis is an important cause of it. Puerariae lobatae Radix (PLR) is a medicine and food homologous species; however, its effect on PE is unclear. This study aimed to investigate the efficacy of PLR in alleviating PE and its mechanisms. We used an NG-nitro-L-arginine methyl ester (L-NAME)-induced PE mouse model to examine the efficacy of preventive and therapeutic PLR supplementation. The results showed that both PLR interventions alleviated hypertension and proteinuria, increased fetal and placental weights, and elevated the levels of VEGF and PlGF. Moreover, PLR protected the placenta from oxidative stress via activating the Nrf2/HO-1/NQO1 pathway and mitigated placental damage by increasing intestinal barrier markers (ZO-1, Occludin, and Claudin-1) expression and reducing lipopolysaccharide leakage. Notably, preventive PLR administration corrected gut dysbiosis in PE mice, as evidenced by the increased abundance and positive interactions of beneficial bacteria including Bifidobacterium, Blautia, and Turicibacter. Fecal microbiota transplantation confirmed that the gut microbiota partially mediated the beneficial effects of PLR on PE. Our findings revealed that modulating the gut microbiota is an effective strategy for the treatment of PE and highlighted that PLR might be used as an intestinal nutrient supplement in PE patients.
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影响因子：
3.5
作者：
Huang L;Jiang Z;Cai R;Li L;Chen Q;Hong J;Hao Z;Wei H
通讯作者：
Wei H
影响因子：
3.9
作者：
Chen L;Jiang E;Guan Y;Xu P;Shen Q;Liu Z;Zhu W;Chen L;Liu H;Dong H
通讯作者：
Dong H
影响因子：
12.2
作者：
Holscher HD
通讯作者：
Holscher HD
DOI：
10.1046/j.1525-1373.1999.d01-139.x
发表时间：
1999-12-01
影响因子：
--
作者：
Hubel, CA
通讯作者：
Hubel, CA
DOI：
10.1016/j.bbadis.2019.165535
发表时间：
2020-02-01
影响因子：
6.2
作者：
Carrasco-Wong, Ivo;Moller, Alejandra;San Martin, Sebastian
通讯作者：
San Martin, Sebastian