Top down proteomics of human membrane proteins from enriched mitochondrial fractions.
Top down proteomics of human membrane proteins from enriched mitochondrial fractions.
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DOI：
10.1021/ac3031527
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发表时间：
2013-02-05
影响因子：
7.4
通讯作者：
Kelleher, Neil L.
中科院分区：
文献类型：
--
作者：
Catherman, Adam D.;Li, Mingxi;Tran, John C.;Durbin, Kenneth R.;Compton, Philip D.;Early, Bryan P.;Thomas, Paul M.;Kelleher, Neil L.
完整完整膜蛋白的鉴定一直是生物质谱技术面临的挑战。在这里，我们展示了自顶向下质谱法在60 kDa以下具有多达8个跨膜螺旋的全膜蛋白中的应用。对人细胞富集的线粒体膜制剂进行分析，鉴定出83种完整膜蛋白，以及163种膜相关或可溶性蛋白，中位q值为3 × 10−10。对匹配片段离子的分析表明，在跨膜结构域中发现的片段离子明显多于根据观察到的蛋白质序列所预期的片段离子。从氧化磷酸化复合体中鉴定出46种蛋白质，这证明了自顶向下蛋白质组学在生物网络中提供广泛覆盖的能力日益增强。
The interrogation of intact integral membrane proteins has long been a challenge for biological mass spectrometry. Here, we demonstrate the application of Top Down mass spectrometry to whole membrane proteins below 60 kDa with up to 8 transmembrane helices. Analysis of enriched mitochondrial membrane preparations from human cells yielded identification of 83 integral membrane proteins, along with 163 membrane-associated or soluble proteins, with a median q value of 3 × 10−10. An analysis of matching fragment ions demonstrated that significantly more fragment ions were found within transmembrane domains than would be expected based upon the observed protein sequence. Forty-six proteins from the complexes of oxidative phosphorylation were identified which exemplifies the increasing ability of Top Down Proteomics to provide extensive coverage in a biological network.
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影响因子：
14.8
作者：
Frezza, Christian;Cipolat, Sara;Scorrano, Luca
通讯作者：
Scorrano, Luca
DOI：
10.1016/j.jasms.2009.08.001
发表时间：
2009-12
影响因子：
3.2
作者：
Lee JE;Kellie JF;Tran JC;Tipton JD;Catherman AD;Thomas HM;Ahlf DR;Durbin KR;Vellaichamy A;Ntai I;Marshall AG;Kelleher NL
通讯作者：
Kelleher NL
影响因子：
3.4
作者：
Durbin, Kenneth R.;Tran, John C.;Zamdborg, Leonid;Sweet, Steve M. M.;Catherman, Adam D.;Lee, Ji Eun;Li, Mingxi;Kellie, John F.;Kelleher, Neil L.
通讯作者：
Kelleher, Neil L.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
7.4
作者：
Kellie, John F.;Catherman, Adam D.;Kelleher, Neil L.
通讯作者：
Kelleher, Neil L.