Diversity of P-element piRNA production among M' and Q strains and its association with P-M hybrid dysgenesis in Drosophila melanogaster.
Diversity of P-element piRNA production among M' and Q strains and its association with P-M hybrid dysgenesis in Drosophila melanogaster.
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DOI：
10.1186/s13100-017-0096-x
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发表时间：
2017
期刊：
影响因子：
4.9
通讯作者：
Itoh M
中科院分区：
文献类型：
--
作者：
Wakisaka KT;Ichiyanagi K;Ohno S;Itoh M
基因组中 P 元件的转座导致果蝇 P-M 杂种发育不全。对于 P 品系，P-M 表型与表达一类小 RNA 的能力相关，这些小 RNA 称为 piwi 相互作用小 RNA (piRNA)，可抑制雌性性腺中的 P 元件。然而，人们对 piRNA 在多大程度上参与 M' 和 Q 菌株的 P-M 杂种发育不全知之甚少，它们在调节 P 菌株的 P 元件方面表现出不同的能力。为了阐明抑制父系遗传 P 元件的分子基础，我们分析了 P 品系的雄性和 M' 或 Q 品系的九系雌性（M' 或 Q 后代）之间 F1 后代中 P 元件的 mRNA 和 piRNA 水平。 M'后代表现出杂种发育不全表型，而Q后代则没有。一致地，M'后代的卵巢和F1胚胎中P元件mRNA的水平高于Q后代，表明M'后代抑制P元件表达的能力较弱。 F1 胚胎中 P 元件 mRNA 的水平与 piRNA 的水平呈负相关。重要的是，M’后代的特点是年轻卵巢和 F1 胚体中 P 元件 piRNA 的丰度较低。尽管所有 Q 后代都抑制其性腺中的 P 元件转座，但 Q 后代在年轻卵巢和 F1 胚胎体中均显示出不同水平的 piRNA。我们的结果与以下观点一致：P 元件 piRNA 的水平是区分 M' 和 Q 菌株类型的决定因素，并且转座元件（包括 piRNA）的抑制机制在自然群体之间存在差异。本文的在线版本 (10.1186/s13100-017-0096-x) 包含补充材料，可供授权用户使用。
Transposition of P elements in the genome causes P–M hybrid dysgenesis in Drosophila melanogaster. For the P strain, the P–M phenotypes are associated with the ability to express a class of small RNAs, called piwi-interacting small RNAs (piRNAs), that suppress the P elements in female gonads. However, little is known about the extent to which piRNAs are involved in the P–M hybrid dysgenesis in M′ and Q strains, which show different abilities to regulate the P elements from P strains. To elucidate the molecular basis of the suppression of paternally inherited P elements, we analyzed the mRNA and piRNA levels of P elements in the F1 progeny between males of a P strain and nine-line females of M′ or Q strains (M′ or Q progenies). M′ progenies showed the hybrid dysgenesis phenotype, while Q progenies did not. Consistently, the levels of P-element mRNA in both the ovaries and F1 embryos were higher in M′ progenies than in Q progenies, indicating that the M′ progenies have a weaker ability to suppress P-element expression. The level of P-element mRNA was inversely correlated to the level of piRNAs in F1 embryos. Importantly, the M′ progenies were characterized by a lower abundance of P-element piRNAs in both young ovaries and F1 embryonic bodies. The Q progenies showed various levels of piRNAs in both young ovaries and F1 embryonic bodies despite all of the Q progenies suppressing P-element transposition in their gonad. Our results are consistent with an idea that the level of P-element piRNAs is a determinant for dividing strain types between M′ and Q and that the suppression mechanisms of transposable elements, including piRNAs, are varied between natural populations. The online version of this article (10.1186/s13100-017-0096-x) contains supplementary material, which is available to authorized users.
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