Thyrotropin-releasing hormone-containing axons innervate histaminergic neurons in the tuberomammillary nucleus.
Thyrotropin-releasing hormone-containing axons innervate histaminergic neurons in the tuberomammillary nucleus.
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DOI：
10.1016/j.brainres.2012.10.010
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发表时间：
2012-12-07
期刊：
影响因子：
2.9
通讯作者：
Fekete C
中科院分区：
文献类型：
--
作者：
Sárvári A;Farkas E;Kádár A;Zséli G;Füzesi T;Lechan RM;Fekete C
最近的研究表明，促甲状腺激素释放激素(TRH)对食物摄入量的调节作用可能是由组胺能神经元介导的。为了阐明TRH和组胺合成神经系统之间功能关系的解剖学基础，对组胺能神经元专属定位的大鼠结节乳头核(TMN)进行了双标记免疫细胞化学研究。组胺能神经元的TRH免疫反应阳性神经支配在TMN的5个亚核(E1-5)均可见，但以E4和E5亚核最为明显，与100%的组胺能神经元接触。与组胺免疫反应神经元接触的TRH-IR阳性神经元数目也以E4和E5亚核最多，E4亚核平均为27.0±1.2，E5亚核平均为7.9±0.5。在E_2和E_3，组胺免疫反应神经元被TRH-IR变异体并列排列，并分别与6.3±0.2和6.8±0.2个变异体/神经支配细胞接触。在E1亚核，TRH阳性轴突与组胺阳性神经元并列的数目最少(85.7±0.9%；4.0±0.2个/神经支配细胞)。超微结构分析表明，TRH-IR轴突在组胺-IR神经元的核周和树突上既存在非对称型突触，也存在对称型突触，但大多数突触为非对称型突触。这些结果表明，TRH神经元大量支配TMN各亚区的组胺能神经元，其中以E4和E5亚区的分布最为密集，并可能发挥激活作用。
Recent studies indicate that the effect of thyrotropin-releasing hormone (TRH) on the regulation of food intake may be mediated by histaminergic neurons. To elucidate the anatomical basis for a functional relationship between TRH- and histamine-synthesizing neuronal systems, double-labeling immunocytochemistry was performed on the tuberomammillary nucleus (TMN) of rats, the exclusive location of histaminergic neurons. TRH-immunoreactive (IR) innervation of the histaminergic neurons were detected in all five subnuclei (E1-5) of the TMN, but was most prominent in the E4 and E5 subnuclei where 100% of the histamine-IR neurons were contacted. The number of TRH-IR varicosities in contact with histamine-IR neurons was also greatest in the E4 and E5 subnuclei, averaging 27.0±1.2 in E4 and 7.9±0.5 in E5. Somewhat fewer histamine-IR neurons were juxtaposed by TRH-IR varicosities in E2 and E3 and contacted by 6.3 ± 0.2 and 6.8 ± 0.2 varicosities/innervated cell, respectively. The number of juxtapositions of TRH-IR axon varicosities with histamine-IR neurons was the lowest in the E1 subnucleus (85.7±0.9%; 4.0±0.2 varicosities/innervated cell). Ultrastructural analysis demonstrated that TRH-IR axons established both asymmetric and symmetric type synapses on the perikaryon and dendrites of the histamine-IR neurons, although the majority of synapses were asymmetric type. These data demonstrate that TRH neurons heavily innervate histaminergic neurons in all subdivisions of the TMN, with the densest innervation in the E4 and E5 subdivisions, and are likely to exert activating effects.
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下丘脑神经元组胺介导了甲状腺蛋白释放激素诱导的食物摄入抑制。
影响因子：
4.7
作者：
Gotoh, Koro;Fukagawa, Koji;Fukagawa, Tomiyo;Noguchi, Hitoshi;Kakuma, Tetsuya;Sakata, Toshiie;Yoshimatsu, Hironobu
通讯作者：
Yoshimatsu, Hironobu
影响因子：
2.5
作者：
BRANCHEREAU, P;VANBOCKSTAELE, EJ;PICKEL, VM
通讯作者：
PICKEL, VM
影响因子：
2.5
作者：
ERICSON, H;WATANABE, T;KOHLER, C
通讯作者：
KOHLER, C
影响因子：
4.1
作者：
Jorgensen, Emilie A.;Vogelsang, Thomas W.;Kjaer, Andreas
通讯作者：
Kjaer, Andreas
影响因子：
2.5
作者：
ITOH, Y;OISHI, R;SAEKI, K
通讯作者：
SAEKI, K