Tracing the mass flow from glucose and phenylalanine to pinoresinol and its glycosides in Phomopsis sp. XP-8 using stable isotope assisted TOF-MS
Tracing the mass flow from glucose and phenylalanine to pinoresinol and its glycosides in Phomopsis sp. XP-8 using stable isotope assisted TOF-MS
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追踪拟茎点霉中从葡萄糖和苯丙氨酸到松脂醇及其糖苷的质量流。
DOI：
10.1038/s41598-019-54836-1
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发表时间：
2019-12
影响因子：
4.6
通讯作者：
Gao Zhenhong
中科院分区：
文献类型：
--
作者：
Zhang Yan;Shi Junling;Ni Yongqing;Liu Yanlin;Zhao Zhixia;Zhao Xixi;Gao Zhenhong
拟茎点霉（Phomopsis sp. XP-8）是杜仲（Eurocumulmoides Oliv）树皮内生真菌，具有以葡萄糖（glu）和苯丙氨酸（Phe）为原料合成松醇（Pin）和松醇二葡萄糖苷（PDG）的能力。为了验证生物合成途径中的质量流，将[13 C6]-标记的glu和[13 C6]-标记的Phe分别作为唯一底物进料至菌株，并通过超高效液相色谱-四极杆飞行时间质谱法检测[13 C6]-标记的产物。结果表明，[13 C6]标记的Phe与[13 C6]-肉桂酸（Ca）和对香豆酸（p-Co）以及[13 C12]标记的Pin结合，表明Pin苯环是通过苯丙烷途径由Phe合成的。[13 C6]标记的葡萄糖可产生[13 C12]标记的Ca和p-Co、[13 C18]标记的Pin、[13 C18]标记的松醇单葡萄糖甙（PMG）和[13 C18]标记的PDG产物，证明PDG的苯环和葡萄糖甙来源于葡萄糖。还确定PMG不是生物合成途径中PDG的直接前体。本研究在质量流水平上鉴定了真菌中苯丙氨酸-木脂素生物合成途径的发生。
Phomopsis sp. XP-8, an endophytic fungus from the bark of Tu-Chung (Eucommia ulmoides Oliv) showed capability to biosynthesize pinoresinol (Pin) and pinoresinol diglucoside (PDG) from glucose (glu) and phenylalanine (Phe). To verify the mass flow in the biosynthesis pathway, [13C6]-labeled glu and [13C6]-labeled Phe were separately fed to the strain as sole substrates and [13C6]-labeled products were detected by ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry. As results, [13C6]-labeled Phe was incorporated into [13C6]-cinnamylic acid (Ca) and p-coumaric acid (p-Co), and [13C12]-labeled Pin, which revealed that the Pin benzene ring came from Phe via the phenylpropane pathway. [13C6]-Labeled Ca and p-Co, [13C12]-labeled Pin, [13C18]-labeled pinoresinol monoglucoside (PMG), and [13C18]-labeled PDG products were found when [13C6]-labeled glu was used, demonstrating that the benzene ring and glucoside of PDG originated from glu. It was also determined that PMG was not the direct precursor of PDG in the biosynthetic pathway. The study identified the occurrence of phenylalanine- lignan biosynthesis pathway in fungi at the level of mass flow.
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影响因子：
4.1
作者：
Yan Zhang;Junling Shi;Zhenhong Gao;J. Che;D. Shao;Yanlin Liu
通讯作者：
Yan Zhang;Junling Shi;Zhenhong Gao;J. Che;D. Shao;Yanlin Liu
影响因子：
5.1
作者：
Pastor V;Sánchez-Bel P;Gamir J;Pozo MJ;Flors V
通讯作者：
Flors V
影响因子：
7
作者：
Yahan Zhou;Jiahong Ma;Jiao Xie;Lili Deng;S. Yao;K. Zeng
通讯作者：
Yahan Zhou;Jiahong Ma;Jiao Xie;Lili Deng;S. Yao;K. Zeng
拟茎点霉 XP-8 静息细胞在苯丙素途径中对底物中的松脂醇二葡萄糖苷和松脂醇进行生物转化
影响因子：
3.7
作者：
Zhang Y;Shi J;Liu L;Gao Z;Che J;Shao D;Liu Y
通讯作者：
Liu Y
DOI：
10.13343/j.cnki.wsxb.20170604
发表时间：
2018-05
期刊：
--
影响因子：
--
作者：
Zhenhong Gao;Zhiwei Zhang;Xiaoguang Xu;J. Che;Yan Zhang;Yanlin Liu;Junling Shi
通讯作者：
Zhenhong Gao;Zhiwei Zhang;Xiaoguang Xu;J. Che;Yan Zhang;Yanlin Liu;Junling Shi