Modulation of LINE-1 and Alu/SVA retrotransposition by Aicardi-Goutières syndrome-related SAMHD1.
Modulation of LINE-1 and Alu/SVA retrotransposition by Aicardi-Goutières syndrome-related SAMHD1.
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DOI：
10.1016/j.celrep.2013.08.019
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发表时间：
2013-09-26
期刊：
影响因子：
8.8
通讯作者：
Yu XF
中科院分区：
文献类型：
--
作者：
Zhao K;Du J;Han X;Goodier JL;Li P;Zhou X;Wei W;Evans SL;Li L;Zhang W;Cheung LE;Wang G;Kazazian HH Jr;Yu XF
Long interspersed elements 1 (LINE-1) occupy at least 17% of the human genome and are its only active autonomous retrotransposons. However, the host factors that regulate LINE-1 retrotransposition are not fully understood. Here, we demonstrate that the Aicardi-Goutières syndrome gene product SAMHD1, recently revealed to be an inhibitor of HIV/simian immunodeficiency virus (SIV) infectivity and neutralized by the viral Vpx protein, is also a potent regulator of LINE-1 and LINE-1-mediated Alu/SVA retrotransposition. We also found that mutant SAMHD1s of Aicardi-Goutières syndrome patients are defective in LINE-1 inhibition. Several domains of SAMHD1 are critical for LINE-1 regulation. SAMHD1 inhibits LINE-1 retrotransposition in dividing cells. An enzymatic active site mutant SAMHD1 maintained substantial anti-LINE-1 activity. SAMHD1 inhibits ORF2p-mediated LINE-1 reverse transcription in isolated LINE-1 ribonucleoproteins by reducing ORF2p level. Thus, SAMHD1 may be a cellular regulator of LINE-1 activity that is conserved in mammals.
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影响因子：
4
作者：
Ali, M;Highet, LJ;Crow, YJ
通讯作者：
Crow, YJ
DOI：
10.1146/annurev-genom-082509-141802
发表时间：
2011
影响因子：
8.7
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影响因子：
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4
作者：
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通讯作者：
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DOI：
10.1073/pnas.0831042100
发表时间：
2003-04-29
影响因子：
11.1
作者：
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通讯作者：
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