From the lab to the field: handheld surface enhanced Raman spectroscopy (SERS) detection of viral proteins.
From the lab to the field: handheld surface enhanced Raman spectroscopy (SERS) detection of viral proteins.
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DOI：
10.1039/d3sd00111c
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发表时间：
2023-11-09
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--
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将传感器从实验室工作台转换为随时可用的需求点设置对于许多领域都是可取的，包括医学，农业和工业。然而，这种转换通常会受到灵敏度损失、高背景信号和其他可能影响再现性的问题的影响。在这里，我们将无标签表面增强拉曼光谱（SERS）传感器用于SARS-CoV-2抗原，从实验室检测应用到手持设备。利用先前用于表面分析的肽捕获分子，我们优化了一种更简单、更具成本效益的基于纳米颗粒的分析。这种新的检测方法允许通过SERS直接检测这些病毒抗原，现在具有鲁棒性和可移植性的优点。我们强调对纳米粒子修饰条件的考虑，并警告可能干扰准确检测的方法。这两种检测方法的比较将有助于指导进一步将基于sers的传感器开发成易于在护理点环境中使用的设备，例如急诊室护士、农民或质量控制技术人员。本研究采用多肽修饰的金纳米颗粒开发了一种可现场检测的SERS传感器，用于使用手持平台直接检测病毒抗原。
Translating sensors from the lab benchtop to a readily available point-of-need setting is desirable for many fields, including medicine, agriculture, and industry. However, this transition generally suffers from loss of sensitivity, high background signals, and other issues which can impair reproducibility. Here we adapt a label-free surface-enhanced Raman spectroscopy (SERS) sensor for SARS-CoV-2 antigens from a lab-based assay to a handheld device. Utilizing a peptide capture molecule, which we previously employed for a surface-based assay, we optimize a simpler and more cost-efficient nanoparticle-based assay. This new assay allows for the direct detection of these viral antigens by SERS, now with the advantages of robustness and portability. We highlight considerations for nanoparticle modification conditions and warn against methods which can interfere with accurate detection. The comparison of these two assays will help guide further development of SERS-based sensors into devices that can be easily used in point-of-care settings, such as by emergency room nurses, farmers, or quality control technicians. This work employs peptide-modified gold nanoparticles to develop a fieldable SERS sensor for direct detection of viral antigens using a handheld platform.
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