Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors.
Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors.
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DOI：
10.1083/jcb.201001035
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发表时间：
2010-07-26
期刊：
影响因子：
--
通讯作者：
Black BE
中科院分区：
文献类型：
--
作者：
Bassett EA;Wood S;Salimian KJ;Ajith S;Foltz DR;Black BE
极光B活性被抑制时，着丝粒重复序列的缺失，虽然动粒仍然可以组装。真核生物中几乎普遍存在的重复着丝粒DNA序列与其明显的功能可分配性形成了矛盾的对比。着丝粒染色质在空间上划分为着丝粒蛋白A（CENP-A）的核小体和内部着丝粒异染色质，缺乏CENP-A，但招募极光B激酶，这是必要的纠正错误的附件有丝分裂纺锤体的着丝粒蛋白A（CENP-A）形成的运动编排阵列。我们发现，在动粒基础上的CENP自我永存网络在缺乏重复α-卫星DNA的人类新着丝粒上是完整的。然而，极光B是不适当的沉默的结果，改变几何形状的新着丝粒，从而损害了纠错机制。这表明了一个模型，其中新着丝粒代表原始遗传基因座，其需要随后产生稳健的内着丝粒区室以增强染色体传递的保真度。
Aurora B activity is inhibited when centromeric repeat sequences are absent, although kinetochores can still assemble. The nearly ubiquitous presence of repetitive centromere DNA sequences across eukaryotic species is in paradoxical contrast to their apparent functional dispensability. Centromeric chromatin is spatially delineated into the kinetochore-forming array of centromere protein A (CENP-A)–containing nucleosomes and the inner centromeric heterochromatin that lacks CENP-A but recruits the aurora B kinase that is necessary for correcting erroneous attachments to the mitotic spindle. We found that the self-perpetuating network of CENPs at the foundation of the kinetochore is intact at a human neocentromere lacking repetitive α-satellite DNA. However, aurora B is inappropriately silenced as a consequence of the altered geometry of the neocentromere, thereby compromising the error correction mechanism. This suggests a model wherein the neocentromere represents a primordial inheritance locus that requires subsequent generation of a robust inner centromere compartment to enhance fidelity of chromosome transmission.
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影响因子：
21.3
作者：
Carroll, Christopher W.;Silva, Mariana C. C.;Godek, Kristina M.;Jansen, Lars E. T.;Straight, Aaron F.
通讯作者：
Straight, Aaron F.
影响因子：
7.5
作者：
Kelly AE;Funabiki H
通讯作者：
Funabiki H
影响因子：
56.9
作者：
Murphy, WJ;Larkin, DM;Lewin, HA
通讯作者：
Lewin, HA
影响因子：
7.5
作者：
Black, Ben E.;Bassett, Emily A.
通讯作者：
Bassett, Emily A.
DOI：
10.1073/pnas.0308637101
发表时间：
2004-04-27
影响因子：
11.1
作者：
Amor, DJ;Bentley, K;Choo, KHA
通讯作者：
Choo, KHA