Analysis of expressed sequence tags and identification of genes encoding cell-wall-degrading enzymes from the fungivorous nematode Aphelenchus avenae.
Analysis of expressed sequence tags and identification of genes encoding cell-wall-degrading enzymes from the fungivorous nematode Aphelenchus avenae.
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DOI：
10.1186/1471-2164-10-525
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发表时间：
2009-11-16
期刊：
影响因子：
4.4
通讯作者：
Kikuchi T
中科院分区：
文献类型：
--
作者：
Karim N;Jones JT;Okada H;Kikuchi T
食真菌线虫，麦长管线虫广泛存在于土壤中，并与腐烂的植物材料有关。这种线虫也被发现与植物有关，但其引起植物疾病的能力在很大程度上尚未确定。本文还讨论了A.燕麦线虫是研究线虫类植物寄生性进化的重要模式。此外，这种线虫在干燥条件下的特殊生存能力使其成为脱水共生研究的重要系统。因此，表达序列标签（EST）分析可能是有用的，提供了一个初步了解的遗传背景的A。燕麦我们从一个混合阶段A.燕麦cDNA文库5,076个高质量EST的聚类产生了一组2,700个非冗余序列，包括695个重叠群和2,005个单例。比较分析表明，1，567个（58.0%）的簇序列在秀丽隐杆线虫中具有同源性，1，750个（64.8%）在其他线虫中，1，321个（48.9%）在线虫以外的生物中，862个（31.9%）与当前蛋白质或核苷酸数据库中的任何序列都没有显著匹配。此外，1,100（40.7%）的序列进行了功能分类使用基因本体论（GO）层次。对簇序列的相似性搜索鉴定了一组与编码降解植物或真菌细胞壁的酶的基因具有显著同源性的基因。鉴定和表征了编码糖基水解酶家族5（GHF 5）纤维素酶的两个基因和编码多糖裂解酶家族3（PL 3）蛋白的两个果胶酸裂解酶基因的全长序列。我们已经描述了至少2，214个来自A.并鉴定了一组编码一系列细胞壁降解酶的基因。该EST数据集代表了许多不同基础和应用领域研究的起点。在A.麦长管蚜和它们与来自其它植物寄生线虫的基因的相似性表明，该线虫不仅可以作为真菌饲养者，而且可以作为植物寄生物。进一步研究了A.麦长管蚜的发现将加速我们对植物寄生的复杂进化历史的理解，以及对通过原核生物水平基因转移获得的基因的利用。
The fungivorus nematode, Aphelenchus avenae is widespread in soil and is found in association with decaying plant material. This nematode is also found in association with plants but its ability to cause plant disease remains largely undetermined. The taxonomic position and intermediate lifestyle of A. avenae make it an important model for studying the evolution of plant parasitism within the Nematoda. In addition, the exceptional capacity of this nematode to survive desiccation makes it an important system for study of anhydrobiosis. Expressed sequence tag (EST) analysis may therefore be useful in providing an initial insight into the poorly understood genetic background of A. avenae. We present the generation, analysis and annotation of over 5,000 ESTs from a mixed-stage A. avenae cDNA library. Clustering of 5,076 high-quality ESTs resulted in a set of 2,700 non-redundant sequences comprising 695 contigs and 2,005 singletons. Comparative analyses indicated that 1,567 (58.0%) of the cluster sequences had homologues in Caenorhabditis elegans, 1,750 (64.8%) in other nematodes, 1,321(48.9%) in organisms other than nematodes, and 862 (31.9%) had no significant match to any sequence in current protein or nucleotide databases. In addition, 1,100 (40.7%) of the sequences were functionally classified using Gene Ontology (GO) hierarchy. Similarity searches of the cluster sequences identified a set of genes with significant homology to genes encoding enzymes that degrade plant or fungal cell walls. The full length sequences of two genes encoding glycosyl hydrolase family 5 (GHF5) cellulases and two pectate lyase genes encoding polysaccharide lyase family 3 (PL3) proteins were identified and characterized. We have described at least 2,214 putative genes from A. avenae and identified a set of genes encoding a range of cell-wall-degrading enzymes. This EST dataset represents a starting point for studies in a number of different fundamental and applied areas. The presence of genes encoding a battery of cell-wall-degrading enzymes in A. avenae and their similarities with genes from other plant parasitic nematodes suggest that this nematode can act not only as a fungal feeder but also a plant parasite. Further studies on genes encoding cell-wall-degrading enzymes in A. avenae will accelerate our understanding of the complex evolutionary histories of plant parasitism and the use of genes obtained by horizontal gene transfer from prokaryotes.
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影响因子：
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作者：
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