Characterising neovascularisation in fracture healing with laser Doppler and micro-CT scanning.
Characterising neovascularisation in fracture healing with laser Doppler and micro-CT scanning.
复制标题
用激光多普勒和微CT扫描来表征骨折愈合中的新血管性。
DOI：
10.1007/s11517-013-1100-3
复制
发表时间：
2013-10
影响因子：
3.2
通讯作者：
Shefelbine, S. J.
中科院分区：
文献类型：
--
作者：
Macdonald, W.;Shefelbine, S. J.
骨折周围软组织的血运是成功愈合的关键，特别是当髓管中的血管破裂时。这项工作的目的是使用激光多普勒和微型计算机断层扫描(Micro-CT)来表征愈合过程中骨折周围软组织的新生血管形成。32只SD大鼠接受了左侧股骨中段截骨术，用定制的外固定器固定。分别于术后2、4、4、6周各处死5只动物。术前、术后及整个愈合期，采用激光多普勒扫描技术测量骨折及健侧肢体的股骨血流灌注量。处死时，插管注入硅胶造影剂。股骨和邻近软组织的微型CT扫描显示了与骨折区域相关的血管特征和分布。术后血液灌注量立即下降，然后通过骨折区域周围小血管的增殖恢复到比术前更高的水平。多模式成像允许对新生血管过程进行纵向的功能分析和详细的结构分析。
Vascularity of the soft tissues around a bone fracture is critical for successful healing, particularly when the vessels in the medullary canal are ruptured. The objective of this work was to use laser Doppler and micro-computer tomography (micro-CT) scanning to characterise neovascularisation of the soft tissues surrounding the fracture during healing. Thirty-two Sprague–Dawley rats underwent mid-shaft osteotomy of the left femur, stabilised with a custom-designed external fixator. Five animals were killed at each of 2, 4 days, 1, 2, 4 and 6 weeks post-operatively. Femoral blood perfusion in the fractured and intact contralateral limbs was measured using laser Doppler scanning pre- and post-operatively and throughout the healing period. At sacrifice, the common iliac artery was cannulated and infused with silicone contrast agent. Micro-CT scans of the femur and adjacent soft tissues revealed vessel characteristics and distribution in relation to the fracture zone. Blood perfusion dropped immediately after surgery and then recovered to greater than the pre-operative level by proliferation of small vessels around the fracture zone. Multi-modal imaging allowed both longitudinal functional and detailed structural analysis of the neovascularisation process.
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影响因子：
2.8
作者：
Harry, Lorraine E.;Sandison, Ann;Nanchahal, Jagdeep
通讯作者：
Nanchahal, Jagdeep
影响因子：
2.3
作者：
ElMaraghy, AW;Schemitsch, EH;Richards, RR
通讯作者：
Richards, RR
影响因子：
8.3
作者：
Garcia-Sanz, A;Rodriguez-Barbero, A;Romero, JC
通讯作者：
Romero, JC
DOI：
10.1097/01.blo.0000150562.24256.a4
发表时间：
2005-04-01
影响因子：
4.2
作者：
Matsuyama, J;Ohnishi, I;Nakamura, K
通讯作者：
Nakamura, K
影响因子：
2.3
作者：
Bumann, M;Henke, T;Augat, P
通讯作者：
Augat, P