Widely Targeted Metabolomics Analysis of Different Parts of Salsola collina Pall.
Widely Targeted Metabolomics Analysis of Different Parts of Salsola collina Pall.
复制标题
DOI：
10.3390/molecules26041126
复制
发表时间：
2021-02-20
期刊：
影响因子：
--
通讯作者：
Ya H
中科院分区：
文献类型：
--
作者：
Li S;Chen Y;Duan Y;Zhao Y;Zhang D;Zang L;Ya H
猪毛菜是一种传统的治疗高血压、头痛、失眠、便秘和眩晕的中药。然而，只有少数生物活性物质已被确定从S。collina。在这里，S. collina，即L-Sc和R-Sc。采用超高效液相色谱-电喷雾电离-串联质谱法（UPLC-ESI-MS/MS）研究了初级和次级代谢产物。共鉴定出637种推定代谢物，这些代谢物主要分为10个不同的类别。代谢产物的相关分析、系统聚类分析、主成分分析和正交偏最小二乘判别分析结果表明，L-Sc样品与R-Sc样品能明显分离。差异累积代谢物分析显示，L-Sc中的大多数差异初级代谢物显著低于R-Sc。进一步的分析表明，黄酮类化合物是推测的主要抗氧化成分，且大多数推测的抗氧化成分在L-Sc中的相对浓度高于R-Sc。这些结果加深了我们对代谢产物积累的认识，并为S. collina。
Salsola collina Pall has a long history of being used as a traditional medicine to treat hypertension, headache, insomnia, constipation and vertigo. However, only a few biologically active substances have been identified from S. collina. Here, the shoots and roots of S. collina, namely L-Sc and R-Sc, were studied. The primary and secondary metabolites were investigated using ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS). A total of 637 putative metabolites were identified and these metabolites were mainly classified into ten different categories. Correlation analysis, hierarchical clustering analysis, principal component analysis and orthogonal partial least squares discriminant analysis of metabolites showed that the L-Sc samples could be clearly separated from the R-Sc samples. Differential accumulated metabolite analysis revealed that most of differential primary metabolites were significantly lower in the L-Sc than in the R-Sc. Conversely, the major differential secondary metabolites had higher levels in the L-Sc than in the R-Sc. Further analysis indicated that the flavonoids were the major putative antioxidant components and most of putative antioxidant components exhibited higher relative concentrations in the L-Sc than the R-Sc. These results improve our understanding of metabolite accumulation and provide a reference for the study of medicinal value in S. collina.
登录
查看更多内容
一种用于大规模检测、鉴定和定量广泛目标代谢物的新型综合方法：在水稻代谢组学研究中的应用。
影响因子：
27.5
作者：
Chen, Wei;Gong, Liang;Luo, Jie
通讯作者：
Luo, Jie
影响因子：
--
作者：
Priyankar Dey, Priyankar Dey;Chaudhuri, T. K.;Dey, P.
通讯作者：
Dey, P.
影响因子：
3.8
作者：
WOLDU, Y;ABEGAZ, B
通讯作者：
ABEGAZ, B
影响因子：
0.8
作者：
Jin, Yong-Sheng;Du, Jing-Ling;Chen, Hai-Sheng
通讯作者：
Chen, Hai-Sheng
影响因子：
0.8
作者：
Syrchina, A. I.;Chernykh, E. A.;Semenov, A. A.
通讯作者：
Semenov, A. A.