Hydrogel-forming microneedles increase in volume during swelling in skin, but skin barrier function recovery is unaffected.
Hydrogel-forming microneedles increase in volume during swelling in skin, but skin barrier function recovery is unaffected.
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DOI：
10.1002/jps.23921
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发表时间：
2014-05
影响因子：
3.8
通讯作者：
Woolfson, A. David
中科院分区：
文献类型：
--
作者：
Donnelly, Ryan F.;Mooney, Karen;Mccrudden, Maeliosa T. C.;Vicente-Perez, Eva M.;Belaid, Luc;Gonzalez-Vazquez, Patricia;Mcelnay, James C.;Woolfson, A. David
我们描述，第一次，量化的皮肤肿胀和液体吸收的水凝胶形成微针阵列（MN）和皮肤屏障恢复人类志愿者。这种MN由水解的聚（甲基乙烯基醚/马来酸酐）（15%w/w）和交联剂聚（乙二醇）10，000道尔顿（7.5%w/w）的水性共混物制备，通过轻轻的手压将其插入人类志愿者（n = 15）的皮肤中约300 μm的深度。MN在皮肤中膨胀，吸收皮肤间质液，使得它们的质量在皮肤中6小时后增加了约30%。然而，重要的是，皮肤屏障功能在显微针移除后24小时内恢复，无论MN在皮肤中存在多长时间或其体积随着肿胀增加多少。MN诱导的微孔关闭的进一步研究是必要的，因为经皮水分损失测量建议关闭，而光学相干断层扫描表明，MN诱导的微孔没有关闭，甚至24小时后，MN已被删除。没有任何志愿者投诉皮肤反应、不良事件或强烈反对使用MN。贴剂揭除后仅观察到一些轻微红斑，但这总是在48小时内消退，随访时未出现不良事件。
We describe, for the first time, quantification of in-skin swelling and fluid uptake by hydrogel-forming microneedle arrays (MN) and skin barrier recovery in human volunteers. Such MN, prepared from aqueous blends of hydrolysed poly(methylvinylether/maleicanhydride) (15% w/w) and the crosslinker poly(ethyleneglycol) 10,000 daltons (7.5% w/w), were inserted into the skin of human volunteers (n = 15) to depths of approximately 300 μm by gentle hand pressure. The MN swelled in skin, taking up skin interstitial fluid, such that their mass had increased by approximately 30% after 6 hours in skin. Importantly, however, skin barrier function recovered within 24 hours post microneedle removal, regardless of how long the MN had been in skin or how much their volume had increased with swelling. Further research on closure of MN-induced micropores is required, since transepidermal water loss measurements suggested micropore closure, while optical coherence tomography indicated that MN-induced micropores had not closed over, even 24 hours after MN had been removed. There were no complaints of skin reactions, adverse events or strong views against MN use by any of the volunteers. Only some minor erythema was noted after patch removal, although this always resolved within 48 hours and no adverse events were present on follow-up.
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影响因子：
19
作者：
Donnelly, Ryan F.;Singh, Thakur Raghu Raj;Garland, Martin J.;Migalska, Katarzyna;Majithiya, Rita;McCrudden, Cian M.;Kole, Prashant Laxman;Mahmood, Tuan Mazlelaa Tuan;McCarthy, Helen O.;Woolfson, A. David
通讯作者：
Woolfson, A. David
影响因子：
3.7
作者：
Kalluri, Haripriya;Banga, Ajay K.
通讯作者：
Banga, Ajay K.
影响因子：
4.9
作者：
Ghosh P;Brogden NK;Stinchcomb AL
通讯作者：
Stinchcomb AL
影响因子：
3.7
作者：
Brogden, Nicole K.;Banks, Stan L.;Stinchcomb, Audra L.
通讯作者：
Stinchcomb, Audra L.
影响因子：
2
作者：
Fercher, Adolf Friedrich
通讯作者：
Fercher, Adolf Friedrich