Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.
Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.
复制标题
从接种疫苗的健康供体中，针对流感病毒的人类单克隆抗体广泛中和。
DOI：
10.1016/j.bbrc.2009.06.151
复制
发表时间：
2009-09-11
影响因子：
3.1
通讯作者：
Ikuta K
中科院分区：
文献类型：
--
作者：
Kubota-Koketsu R;Mizuta H;Oshita M;Ideno S;Yunoki M;Kuhara M;Yamamoto N;Okuno Y;Ikuta K
从病毒感染患者中制备的人单克隆抗体（HuMAbs）可以提供有关人类表位的信息，这些信息对于疫苗的开发以及潜在的治疗应用至关重要。通过将来自总共5名流感疫苗接种志愿者的外周血单个核细胞与新开发的鼠-人嵌合体融合伴侣细胞SPYMEG融合，我们获得了10个稳定产生抗流感病毒抗体的杂交瘤克隆：1个针对甲型H1 N1流感病毒，4个针对甲型H3 N2流感病毒，5个针对B流感病毒。令人惊讶的是，大多数HuMAb在亚型内显示出广泛的反应性，四种（两种针对H3 N2，两种针对B）显示出广泛的中和能力。重要的是，表位作图显示，来自不同供体的两种针对H3 N2的广谱中和抗体识别位于H3 N2毒株之间高度保守的血凝素球状区的受体结合位点下方的相同表位。
Human monoclonal antibodies (HuMAbs) prepared from patients with viral infections could provide information on human epitopes important for the development of vaccines as well as potential therapeutic applications. Through the fusion of peripheral blood mononuclear cells from a total of five influenza-vaccinated volunteers, with newly developed murine–human chimera fusion partner cells, named SPYMEG, we obtained 10 hybridoma clones stably producing anti-influenza virus antibodies: one for influenza A H1N1, four for influenza A H3N2 and five for influenza B. Surprisingly, most of the HuMAbs showed broad reactivity within subtype and four (two for H3N2 and two for B) showed broad neutralizing ability. Importantly, epitope mapping revealed that the two broad neutralizing antibodies to H3N2 derived from different donors recognized the same epitope located underneath the receptor-binding site of the hemagglutinin globular region that is highly conserved among H3N2 strains.
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影响因子：
3.1
作者：
Simmons, C. P.;Bernasconi, N. L.;Suguitan, A. L.
通讯作者：
Suguitan, A. L.
影响因子：
64.8
作者：
Wrammert, Jens;Smith, Kenneth;Wilson, Patrick C.
通讯作者：
Wilson, Patrick C.
影响因子：
64.8
作者：
WILEY, DC;WILSON, IA;SKEHEL, JJ
通讯作者：
SKEHEL, JJ
影响因子：
64.5
作者：
GREEN, N;ALEXANDER, H;LERNER, RA
通讯作者：
LERNER, RA
影响因子：
9.4
作者：
OKUNO, Y;TANAKA, K;UEDA, S
通讯作者：
UEDA, S