Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum.
Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum.
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细长堇草谷醇溶蛋白基因的全基因组鉴定、特征和表达
DOI：
10.1186/s12864-021-08088-x
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发表时间：
2021-12-02
期刊：
影响因子：
4.4
通讯作者：
Sun S
中科院分区：
文献类型：
--
作者：
Ge W;Gao Y;Xu S;Ma X;Wang H;Kong L;Sun S
背景
谷醇溶蛋白是禾本科植物特有的蛋白质，在人类饮食中起着关键作用。长穗偃麦草Thinopyrum elongatum（syn.长穗偃麦草（Agropyron elongatum或Lophopyrum elongatum）是一种具有二倍体E基因组（2n = 2x = 14）的小麦族禾本科牧草，其醇溶蛋白基因及其与小麦族同源基因座的关系尚不清楚。
结果
本研究共检测到19个α-醇溶蛋白基因、9个γ-醇溶蛋白基因、19个ω-醇溶蛋白基因、2个高分子量麦谷蛋白亚基（HMW-GS）基因和5个低分子量麦谷蛋白亚基（LMW-GS）基因。elongatum基因组微同线性和系统发育分析揭示了不同种间醇溶蛋白基因区域的动态变化和遗传亲缘关系。elongatum、Triticum aestivum、T. urartu和节节麦Aegilops tauschii。The Th. elongatum的B亚基因组，与T. aestivum中只含有乳糜泻表位DQ 8-glia-α1/DQ 8. 5-glia-α1，为低筋毒小麦育种提供了理论依据。转录组数据分析表明，Th. elongatum在同一亚科或不同亚科中的表达量和表达模式存在差异。面团流变学特性的研究。aestivum-Th.长穗偃麦草二体代换系1 E（1D）表现出较高的峰高值，DS 6 E（6D）表现出较低的α-醇溶蛋白含量，在小麦品质育种中具有潜在的应用价值。
结论
总之，这项研究提供了一个全面的概述醇溶谷蛋白基因家族在Th。elongatum，并提出了一个有前途的使用这个物种的一代改良小麦品种打算为人类的饮食。
Background
Prolamins, unique to Gramineae (grasses), play a key role in the human diet. Thinopyrum elongatum (syn. Agropyron elongatum or Lophopyrum elongatum), a grass of the Triticeae family with a diploid E genome (2n = 2x = 14), is genetically well-characterized, but little is known about its prolamin genes and the relationships with homologous loci in the Triticeae species.
Results
In this study, a total of 19 α-gliadin, 9 γ-gliadin, 19 ω-gliadin, 2 high-molecular-weight glutenin subunit (HMW-GS), and 5 low-molecular-weight glutenin subunit (LMW-GS) genes were identified in the Th. elongatum genome. Micro-synteny and phylogenetic analysis revealed dynamic changes of prolamin gene regions and genetic affinities among Th. elongatum, Triticum aestivum, T. urartu and Aegilops tauschii. The Th. elongatum genome, like the B subgenome of T. aestivum, only contained celiac disease epitope DQ8-glia-α1/DQ8.5-glia-α1, which provided a theoretical basis for the low gluten toxicity wheat breeding. The transcriptome data of Th. elongatum exhibited differential expression in quantity and pattern in the same subfamily or different subfamilies. Dough rheological properties of T. aestivum-Th. elongatum disomic substitution (DS) line 1E(1D) showed higher peak height values than that of their parents, and DS6E(6D) exhibited fewer α-gliadins, which indicates the potential usage for wheat quality breeding.
Conclusions
Overall, this study provided a comprehensive overview of the prolamin gene family in Th. elongatum, and suggested a promising use of this species in the generation of improved wheat breeds intended for the human diet.
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影响因子：
64.8
作者：
Luo MC;Gu YQ;Puiu D;Wang H;Twardziok SO;Deal KR;Huo N;Zhu T;Wang L;Wang Y;McGuire PE;Liu S;Long H;Ramasamy RK;Rodriguez JC;Van SL;Yuan L;Wang Z;Xia Z;Xiao L;Anderson OD;Ouyang S;Liang Y;Zimin AV;Pertea G;Qi P;Bennetzen JL;Dai X;Dawson MW;Müller HG;Kugler K;Rivarola-Duarte L;Spannagl M;Mayer KFX;Lu FH;Bevan MW;Leroy P;Li P;You FM;Sun Q;Liu Z;Lyons E;Wicker T;Salzberg SL;Devos KM;Dvořák J
通讯作者：
Dvořák J
影响因子：
5.1
作者：
Kong, XY;Gu, YQ;Anderson, OD
通讯作者：
Anderson, OD
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
4.6
作者：
Huo N;Zhu T;Altenbach S;Dong L;Wang Y;Mohr T;Liu Z;Dvorak J;Luo MC;Gu YQ
通讯作者：
Gu YQ
影响因子：
64.8
作者：
Ling HQ;Ma B;Shi X;Liu H;Dong L;Sun H;Cao Y;Gao Q;Zheng S;Li Y;Yu Y;Du H;Qi M;Li Y;Lu H;Yu H;Cui Y;Wang N;Chen C;Wu H;Zhao Y;Zhang J;Li Y;Zhou W;Zhang B;Hu W;van Eijk MJT;Tang J;Witsenboer HMA;Zhao S;Li Z;Zhang A;Wang D;Liang C
通讯作者：
Liang C