MONOPTEROS directly activates the auxin-inducible promoter of the Dof5.8 transcription factor gene in Arabidopsis thaliana leaf provascular cells.
MONOPTEROS directly activates the auxin-inducible promoter of the Dof5.8 transcription factor gene in Arabidopsis thaliana leaf provascular cells.
复制标题
DOI：
10.1093/jxb/eru418
复制
发表时间：
2015-01
影响因子：
6.9
通讯作者：
Yanagisawa S
中科院分区：
文献类型：
--
作者：
Konishi M;Donner TJ;Scarpella E;Yanagisawa S
拟南芥基因 Dof5.8 是生长素响应性 MONOPTEROS 转录因子的靶标。当与单翅目等位基因结合时，Dof5.8 的突变会干扰胚胎发育和静脉图案。 MONOPTEROS (MP) 是一种生长素响应性转录因子，是初生根形成和维管发育所需的，而 Dof5.8 是一种 Dof 类转录因子，其基因在拟南芥的胚胎以及叶原基的前形成层和原形成层细胞中表达。本研究表明 MP 直接激活 Dof5.8 启动子。虽然没有发现单个 dof5.8 突变体的明显表型，但对 mp dof5.8 双突变体的表型分析表明，Dof5.8 内的突变增强了 mp 弱等位基因的表型，“无根”表型的外显率增加，子叶数量减少。此外，有趣的是，尽管 mp 突变体显示子叶中维管模式复杂性降低，但 mp dof5.8 双突变体在单个子叶中显示出更简单和更复杂的维管模式。这些结果暗示其表达至少部分受MP调节的Dof5.8的产物可能参与由MP控制的多个过程。
An Arabidopsis gene, Dof5.8, is a target of the auxin-responsive MONOPTEROS transcription factor. Mutations in Dof5.8 interfere with embryo development and vein patterning when combined with a monopteros allele. MONOPTEROS (MP) is an auxin-responsive transcription factor that is required for primary root formation and vascular development, whereas Dof5.8 is a Dof-class transcription factor whose gene is expressed in embryos as well as the pre- and procambial cells in the leaf primordium in Arabidopsis thaliana. In this study, it is shown that MP directly activates the Dof5.8 promoter. Although no apparent phenotype of the single dof5.8 mutants was found, phenotypic analysis with the mp dof5.8 double mutants revealed that mutations within Dof5.8 enhanced the phenotype of a weak allele of mp, with an increase in the penetrance of the ‘rootless’ phenotype and a reduction in the number of cotyledons. Furthermore, interestingly, although mp mutants showed reduced vascular pattern complexity in cotyledons, the mp dof5.8 double mutants displayed both more simplex and more complex vascular patterns in individual cotyledons. These results imply that the product of Dof5.8 whose expression is regulated by MP at least in part might be involved in multiple processes controlled by MP.
登录
查看更多内容
影响因子：
11.6
作者：
Guo, Yong;Qin, Genji;Qu, Li-Jia
通讯作者：
Qu, Li-Jia
影响因子：
4.6
作者：
Hardtke, CS;Ckurshumova, W;Berleth, T
通讯作者：
Berleth, T
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
6.5
作者：
Konishi, Mineko;Yanagisawa, Shuichi
通讯作者：
Yanagisawa, Shuichi
影响因子：
2.7
作者：
Donnelly, PM;Bonetta, D;Dengler, NG
通讯作者：
Dengler, NG