Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques.
Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques.
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DOI：
10.1038/s41598-017-05554-z
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发表时间：
2017-07-12
影响因子：
4.6
通讯作者：
Zhu LG
中科院分区：
文献类型：
--
作者：
Zou Y;Li J;Cui Y;Tang P;Du L;Chen T;Meng K;Liu Q;Feng H;Zhao J;Chen M;Zhu LG
虽然中枢神经系统髓磷脂缺陷导致几种严重疾病，但缺乏明确的诊断手段。我们提出并实施了太赫兹时域光谱（THz-TDS）结合化学计量技术来区分和评估小鼠和恒河猴大脑髓磷脂缺陷的严重程度。用石蜡包埋法测定了正常小鼠和突变小鼠脑组织的太赫兹折射率和吸收系数。时域太赫兹信号的主成分分析（PCA-tdTHz）和太赫兹光谱的吸收-折射率关系无需外源标记或任何预处理即可识别髓磷脂缺陷。此外，我们利用建立的PCA-tdTHz，评估了研究最多的多发性硬化症动物模型——实验性自身免疫性脑脊髓炎（实验性自身免疫性脑脊髓炎）对恒河猴脑髓鞘缺损病变的严重程度。结果与病理分析相吻合，表明PCA-tdTHz是一种快速、强大、不断发展的工具，可用于鉴定和评估临床前动物和准临床人类活检中的髓磷脂缺陷。
While myelin deficit of the central nervous system leads to several severe diseases, the definitive diagnostic means are lacking. We proposed and performed terahertz time-domain spectroscopy (THz-TDS) combined with chemometric techniques to discriminate and evaluate the severity of myelin deficit in mouse and rhesus monkey brains. The THz refractive index and absorption coefficient of paraffin-embedded brain tissues from both normal and mutant dysmyelinating mice are shown. Principal component analysis of time-domain THz signal (PCA-tdTHz) and absorption-refractive index relation of THz spectrum identified myelin deficit without exogenous labeling or any pretreatment. Further, with the established PCA-tdTHz, we evaluated the severity of myelin deficit lesions in rhesus monkey brain induced by experimental autoimmune encephalomyelitis, which is the most-studied animal model of multiple sclerosis. The results well matched the pathological analysis, indicating that PCA-tdTHz is a quick, powerful, evolving tool for identification and evaluation myelin deficit in preclinical animals and potentially in para-clinical human biopsy.
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影响因子：
3.4
作者：
Oh SJ;Kim SH;Ji YB;Jeong K;Park Y;Yang J;Park DW;Noh SK;Kang SG;Huh YM;Son JH;Suh JS
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Debbage, P
影响因子：
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作者：
Shi, Lingyan;Shumyatsky, Pavel;Alfano, Robert
通讯作者：
Alfano, Robert
影响因子：
11.2
作者：
Polman CH;Reingold SC;Banwell B;Clanet M;Cohen JA;Filippi M;Fujihara K;Havrdova E;Hutchinson M;Kappos L;Lublin FD;Montalban X;O'Connor P;Sandberg-Wollheim M;Thompson AJ;Waubant E;Weinshenker B;Wolinsky JS
通讯作者：
Wolinsky JS