Expression of Mug14 is regulated by the transcription factor Rst2 through the cAMP-dependent protein kinase pathway in Schizosaccharomyces pombe
Expression of Mug14 is regulated by the transcription factor Rst2 through the cAMP-dependent protein kinase pathway in Schizosaccharomyces pombe
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粟酒裂殖酵母中 Mug14 的表达受转录因子 Rst2 通过 cAMP 依赖性蛋白激酶途径的调节
DOI：
10.1007/s00294-021-01194-z
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发表时间：
2021
期刊：
影响因子：
2.5
通讯作者：
Matsuo Yasuhiro
中科院分区：
文献类型：
--
作者：
Inamura Shin-ich;Tanabe Takuma;Kawamukai Makoto;Matsuo Yasuhiro
在粟酒裂殖酵母中，cAMP依赖性蛋白激酶（Pka 1）调节许多细胞事件，包括性发育和糖原生成，以及对葡萄糖限制的反应。尽管它在许多细胞事件中的重要性，cAMP/PKA途径的目标尚未得到充分研究。在这里，我们证明mug 14的表达是由cAMP/PKA通路的下调和葡萄糖的限制诱导的。这种调节依赖于Rst 2的功能，Rst 2是一种调节从有丝分裂到减数分裂转变的转录因子。Rst 2中C2 H2型锌指结构域的丢失，称为Rst 2（C2 H2 β），消除了Mug 14表达的诱导。在S. pka 1 pombe（STREP：CCCCTC）序列，该序列是Rst 2在mug 14上的潜在结合位点，在pka 1 pombe菌株中，其诱导作用被消除。在therst 2菌株中，葡萄糖限制和氮饥饿显著降低和延迟了Mug 14的表达。已知Mug 14与Mde 1和Mta 3在蛋氨酸补救途径中具有共同的功能，但mde 1和mta 3 mRNA的表达并不因ka 1缺失和葡萄糖限制而增强。我们的结论是，Mug 14的表达上调Rst 2的控制下的cAMP/PKA信号通路，这感觉到葡萄糖的限制。
The cAMP-dependent protein kinase (Pka1) regulates many cellular events, including sexual development and glycogenesis, and response to the limitation of glucose, inSchizosaccharomyces pombe. Despite its importance in many cellular events, the targets of the cAMP/PKA pathway have not been fully investigated. Here, we demonstrate that the expression ofmug14is induced by downregulation of the cAMP/PKA pathway and limitation of glucose. This regulation is dependent on the function of Rst2, a transcription factor that regulates transition from mitosis to meiosis. The loss of the C2H2-type zinc finger domain in Rst2, termed Rst2 (C2H2∆), abolished the induction of Mug14 expression. Upon deletion of the stress starvation response element of theS. pombe(STREP: CCCCTC) sequence, which is a potential binding site of Rst2 onmug14, in thepka1∆strain, its induction was abolished. The expression of Mug14 was significantly reduced and delayed by the limitation of glucose and also by nitrogen starvation in therst2∆strain. Mug14 is known to share a common function with Mde1 and Mta3 in the methionine salvage pathway, but the expression ofmde1andmta3mRNAs was not enhanced bypka1deletion and limitation of glucose. We conclude that the expression of Mug14 is upregulated by Rst2 under the control of the cAMP/PKA signaling pathway, which senses the limitation of glucose.
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