Multiplex detection of breast cancer biomarkers using plasmonic molecular sentinel nanoprobes.

Multiplex detection of breast cancer biomarkers using plasmonic molecular sentinel nanoprobes.
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DOI:
10.1088/0957-4484/20/6/065101
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发表时间:
2009-02-11
期刊:
影响因子:
3.5
通讯作者:
Vo-Dinh T
Vo-Dinh T
中科院分区:
材料科学3区
文献类型:
--
作者:
Wang HN;Vo-Dinh T

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我们首次证明了在均相溶液中使用基于表面增强拉曼散射的分子哨兵 (MS) 技术进行多重检测的可行性。使用两种带有不同拉曼标记的 MS 纳米探针来检测 erbB-2 和 ki-67 乳腺癌生物标志物的存在。通过混合两种 MS 纳米探针并在单个或多个 DNA 靶标存在的情况下进行测试,证明了 MS 技术的多重分析能力。
We have demonstrated for the first time the feasibility of multiplex detection using the surface-enhanced Raman scattering-based molecular sentinel (MS) technology in a homogeneous solution. Two MS nanoprobes tagged with different Raman labels were used to detect the presence of the erbB-2 and ki-67 breast cancer biomarkers. The multiplexing capability of the MS technique was demonstrated by mixing the two MS nanoprobes and tested in the presence of single or multiple DNA targets.
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