Murine natural killer cells express functional Fc gamma receptor II encoded by the Fc gamma R alpha gene.
Murine natural killer cells express functional Fc gamma receptor II encoded by the Fc gamma R alpha gene.
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鼠天然杀伤细胞表达由FC伽马α基因编码的功能性FC伽马受体II。
DOI：
10.1084/jem.170.1.73
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发表时间：
1989-07-01
影响因子：
15.3
通讯作者：
KUMAR, V
中科院分区：
文献类型：
--
作者：
PERUSSIA, B;TUTT, MM;QIU, WQ;KUZIEL, WA;TUCKER, PW;TRINCHIERI, G;BENNETT, M;RAVETCH, JV;KUMAR, V
我们报告了小鼠NK细胞表达由Fc γ RII α基因编码的功能性Fc γ RII的证据。几条间接证据表明，从几个品系的小鼠新鲜获得的NK细胞携带功能性Fc γ RII：（a）抗Fc γ RII抗体2.4G2检测到小但显著比例的sIg-细胞，并且小比例的2.4G2+细胞包括在Thy-1+群体中;（B）sIg-淋巴细胞含有相似比例的2.4G 2+和Fc γ R-携带细胞;（c）sIg-淋巴细胞对颗粒免疫复合物的结合被2.4G 2完全抑制;（d）2.4G2+细胞介导大于50%的sIg-脾淋巴细胞中的自发细胞毒性。对C57 BL/6小鼠脾淋巴细胞进行的双色免疫荧光研究结果显示NK-1.1和2.4G2共表达，提供了小鼠NK细胞上存在Fc γ RII的直接证据。体外繁殖的同质NK细胞群的研究证实，鼠NK细胞仅表达Fc γ RII，并且该Fc γ R具有功能性，如抗Fc γ RII抗体2.4G2抑制ADCC的实验所示。分子水平的研究结果表明，与巨噬细胞中表达的Fc γ RII α转录物相同的Fc γ RII α转录物是鼠NK细胞中编码Fc γ RII的唯一分子。
We report evidence that murine NK cells express a functional Fc gamma RII encoded by the Fc gamma RII alpha gene. Several lines of indirect evidence indicate that freshly obtained NK cells from mice of several strains bear a functional Fc gamma RII: (a) anti-Fc gamma RII antibody 2.4G2 detects a small but significant proportion of sIg- cells and a small proportion of the 2.4G2+ cells are included in the Thy-1+ population; (b) sIg- lymphocytes contain 2.4G2+ and Fc gamma R-bearing cells in similar proportions; (c) binding of particulate immune complexes by sIg- lymphocytes is completely inhibited by 2.4G2; (d) 2.4G2+ cells mediate greater than 50% of the spontaneous cytotoxicity in sIg- splenic lymphocytes. Direct evidence for the presence of Fc gamma RII on murine NK cells is provided by the results of two-color immunofluorescence studies performed on splenic lymphocytes from C57BL/6 mice showing coexpression of NK-1.1 and 2.4G2. Studies of in vitro propagated homogeneous NK cell populations confirm that murine NK cells express only Fc gamma RII and that this Fc gamma R is functional, as shown in experiments of inhibition of ADCC by the anti-Fc gamma RII antibody 2.4G2. The results of studies at the molecular level show that an Fc gamma RII alpha transcript identical to that expressed in macrophages is the only molecule encoding Fc gamma RII in murine NK cells.
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影响因子：
15.3
作者：
DIAMOND, B;YELTON, DE
通讯作者：
YELTON, DE
影响因子：
15.9
作者：
KURLANDER, RJ;HALL, J
通讯作者：
HALL, J
影响因子：
64.5
作者：
CLEVELAND, DW;LOPATA, MA;KIRSCHNER, MW
通讯作者：
KIRSCHNER, MW
影响因子：
64.8
作者：
HUIZINGA, TWJ;VANDERSCHOOT, CE;TETTEROO, PAT
通讯作者：
TETTEROO, PAT
影响因子：
5.4
作者：
AUSTYN, JM;GORDON, S
通讯作者：
GORDON, S