Gastric mucosal microbiota in a Mongolian population with gastric cancer and precursor conditions.
Gastric mucosal microbiota in a Mongolian population with gastric cancer and precursor conditions.
复制标题
蒙古人群中胃癌和前体疾病的胃粘膜微生物群。
DOI：
10.1111/apt.15675
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发表时间：
2020-04
影响因子：
7.6
通讯作者：
Yamaoka Y
中科院分区：
文献类型：
--
作者：
Gantuya B;El Serag HB;Matsumoto T;Ajami NJ;Uchida T;Oyuntsetseg K;Bolor D;Yamaoka Y
蒙古国胃癌的发病率和死亡率很高，尽管蒙古国人口中的幽门螺杆菌毒性较低。我们的目的是评估胃癌患者的胃细菌微生物群特征及其前体组织学状况。我们对48例胃癌患者和120例非癌症患者（20例正常胃粘膜[对照]、20例胃炎、40例萎缩和40例肠化[IM]）进行了一项病例对照研究。我们进行了16 S rRNA基因扩增测序，并比较了基于诊断组和H.幽门螺杆菌感染状况。在对照组中观察到最高的总体细菌α多样性指标，其次是IM组和癌症组。胃炎和萎缩组的多样性最小。乳酸杆菌和肠球菌是几种癌症患者的优势菌属，特别是在缺乏H。幽门。此外，肉杆菌属、谷氨酸杆菌属、类谷氨酸杆菌属、梭杆菌属和微小单胞菌属与胃癌相关，与H.幽门感染厚壁菌在胃炎和萎缩组中减少，而在IM和癌症组中增加。与非癌症组相比，癌症组的Embden-Meyerhof-Parnas途径的功能代谢活性和糖的利用显著增加。幽门螺杆菌以外的微生物因素可能在蒙古族胃癌中起作用。我们确定了胃癌与肠球菌属、乳杆菌属、肉杆菌属、谷氨酸杆菌属、类谷氨酸杆菌属、梭杆菌属和细小单胞菌属之间的新关联。
Incidence and mortality of gastric cancer are high in Mongolia despite Helicobacter pylori in the Mongolian population being less virulent. We aimed to evaluate gastric bacterial microbiota profiles in patients with gastric cancer and its precursor histological conditions. We conducted a case-control study among 48 gastric cancer and 120 non-cancer patients (20 normal gastric mucosa [control], 20 gastritis, 40 with atrophy, and 40 intestinal metaplasia [IM]). We performed 16S rRNA gene amplicon sequencing and compared taxonomic and functional prediction profiles based on the diagnosis group and H. pylori infection status. The highest overall bacterial alpha diversity metrics were observed in the control group, followed by the IM and cancer groups. The gastritis and atrophy groups had the least diversity. Lactobacilli and Enterococci were the dominant genus in several cancer patients especially in the absence of H. pylori. In addition, Carnobacterium, Glutamicibacter, Paeniglutamicibacter, Fusobacterium, and Parvimonas were associated with gastric cancer regardless of H. pylori infection. Firmicutes were decreased in the gastritis and atrophy groups and increased in the IM and cancer groups. The functional metabolic activity of the Embden-Meyerhof-Parnas pathway and the utilization of sugar, were significantly increased in cancer group compared with the non-cancer group. Microbial factors other than H pylori may play a role in Mongolian gastric cancer. We identified novel associations between gastric cancer and the genera Enterococcus, Lactobacillus, Carnobacterium, Glutamicibacter, Paeniglutamicibacter, Fusobacterium, and Parvimonas.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
4.6
作者：
Aviles-Jimenez F;Vazquez-Jimenez F;Medrano-Guzman R;Mantilla A;Torres J
通讯作者：
Torres J
影响因子：
24.5
作者：
Rugge, Massimo;Meggio, Alberto;Graham, David Y.
通讯作者：
Graham, David Y.
影响因子：
4.6
作者：
Li TH;Qin Y;Sham PC;Lau KS;Chu KM;Leung WK
通讯作者：
Leung WK
影响因子：
6.7
作者：
Noto JM;Peek RM Jr
通讯作者：
Peek RM Jr