Hepatitis C virus-specific cell-mediated immune responses in children born to mothers infected with hepatitis C virus.
Hepatitis C virus-specific cell-mediated immune responses in children born to mothers infected with hepatitis C virus.
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DOI：
10.1016/j.jpeds.2012.06.057
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发表时间：
2013-01
影响因子：
5.1
通讯作者：
Shata, Mohamed Tarek
中科院分区：
文献类型：
--
作者：
El-Kamary, Samer S.;Hashem, Mohamed;Saleh, Doaa A.;Abdelwahab, Sayed F.;Sobhy, Maha;Shebl, Fatma M.;Shardell, Michelle D.;Strickland, G. Thomas;Shata, Mohamed Tarek
探讨丙型肝炎病毒（HCV）感染母亲所生儿童的HCV特异性细胞介导免疫（CMI）应答与病毒清除之间的关系。一项横断面研究纳入了来自埃及母婴队列的儿童，使用干扰素-γ酶联免疫斑点试验检测对重组核心和非结构HCV抗原（HCV基因组的非结构片段NS 3、NS 4a/B和NS 5）的CMI应答。将患有慢性HCV的母亲所生的儿童纳入3组：一过性病毒血症（n = 5），病毒血症（n = 36）和阳性对照（n = 6），其中包括来自该队列的1名慢性HCV儿童和来自同伴研究的另外5名慢性HCV儿童。无HCV母亲所生的无HCV儿童（n = 27）作为阴性对照组。使用Wilcoxon秩和检验比较组间CMI反应的幅度。6名HCV阳性的对照儿童中没有一名对任何HCV抗原有反应，5名一过性病毒血症儿童中有4名（80%）对至少一种HCV抗原有反应，而在病毒血症组和阴性对照组中，36名儿童中有5名（14%）对至少一种HCV抗原有反应，27名儿童中有3名（11%）对至少一种HCV抗原有反应。与阴性对照组（P = 0.005）、阳性对照组（P = 0.011）或无HCV病毒血症的儿童（P = 0.012）相比，一过性病毒血症的儿童引起更强的反应，特别是对非结构抗原。HCV特异性CMI反应在短暂感染儿童中的幅度和频率显著高于持续感染儿童。这表明CMI反应可能与过去的病毒清除有关，可以识别感染高风险的儿童，这些儿童可以成为健康教育，筛查和随访的目标。
To investigate the association between hepatitis C virus (HCV)-specific cell-mediated immunity (CMI) responses and viral clearance in children born to mothers infected with HCV. A cross-sectional study of children from a mother-infant cohort in Egypt were enrolled to detect CMI responses to recombinant core and nonstructural HCV antigens (nonstructural segments NS3, NS4a/b, and NS5 of the HCV genome) using an interferon-gamma enzyme-linked immunospot assay. Children born to mothers with chronic HCV were enrolled into 3 groups: transiently viremic (n = 5), aviremic (n = 36), and positive control (n = 6), which consisted of 1 child with chronic HCV from this cohort and another 5 children with chronic HCV from a companion study. Children without HCV born to mothers without HCV (n = 27) served as a negative control group. Wilcoxon rank sum test was used to compare the magnitude of CMI responses between groups. None of the 6 control children who were positive for HCV responded to any HCV antigen, and 4 (80%) of 5 children with transient viremia responded to at least one HCV antigen, compared with 5 (14%) of 36 and 3 (11%) of 27 children in the aviremic and negative control groups, respectively. Children with transient viremia elicited stronger responses than did negative controls (P = .005), positive controls (P = .011), or children without HCV viremia (P = .012), particularly to nonstructural antigens. HCV-specific CMI responses were significantly higher in magnitude and frequency among transiently infected children compared with those persistently infected. This suggests CMI responses may be associated with past viral clearance and can identify children at high risk of infection, who can be targeted for health education, screening, and follow-up.
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