3D cell entrapment in crosslinked thiolated gelatin-poly(ethylene glycol) diacrylate hydrogels.
3D cell entrapment in crosslinked thiolated gelatin-poly(ethylene glycol) diacrylate hydrogels.
复制标题
DOI：
10.1016/j.biomaterials.2011.09.031
复制
发表时间：
2012-01
期刊：
影响因子：
14
通讯作者：
Kao, Weiyuan J.
中科院分区：
文献类型：
--
作者：
Fu, Yao;Xu, Kedi;Zheng, Xiaoxiang;Giacomin, Alan J.;Mix, Adam W.;Kao, Weiyuan J.
天然ECM成分和合成材料的结合使用为制造基于水凝胶的组织工程支架提供了一种有吸引力的替代方法，可以在三维（3D）中研究细胞-基质的相互作用。提出了一种用半胱氨酸通过双功能聚乙二醇连接剂修饰明胶的简便方法，从而在明胶链上引入游离巯基。以硫代明胶和聚乙二醇二丙烯酸酯（PEGdA）为原料，通过硫烯反应制备了共价交联明胶水凝胶。未改性的明胶物理掺入PEGdA-only基质进行比较。我们试图了解交联方式对水凝胶物理化学性质的影响以及对三维细胞包裹的影响。与物理掺入的明胶水凝胶相比，共价交联的明胶水凝胶具有更高的最大重量溶胀比（Qmax）、更高的含水量、显著降低的累计明胶溶解（7天）和更低的凝胶硬度。此外，包裹在共价交联明胶水凝胶中的成纤维细胞在28天内显示出广泛的细胞质扩散和细胞网络的形成。相比之下，包被物理纳入明胶水凝胶的成纤维细胞保持球形。因此，将ECM蛋白与合成基质交联可以创建一个稳定的支架，具有可调的机械性能和长期的细胞锚点，从而支持细胞在3D环境中的附着和生长。
The combined use of natural ECM components and synthetic materials offers an attractive alternative to fabricate hydrogel-based tissue engineering scaffolds to study cell-matrix interactions in three-dimensions (3D). A facile method was developed to modify gelatin with cysteine via a bifunctional PEG linker, thus introducing free thiol groups to gelatin chains. A covalently crosslinked gelatin hydrogel was fabricated using thiolated gelatin and poly(ethylene glycol) diacrylate (PEGdA) via thiol-ene reaction. Unmodified gelatin was physically incorporated in a PEGdA-only matrix for comparison. We sought to understand the effect of crosslinking modality on hydrogel physicochemical properties and the impact on 3D cell entrapment. Compared to physically incorporated gelatin hydrogels, covalently crosslinked gelatin hydrogels displayed higher maximum weight swelling ratio (Qmax), higher water content, significantly lower cumulative gelatin dissolution up to 7 days, and lower gel stiffness. Furthermore, fibroblasts encapsulated within covalently crosslinked gelatin hydrogels showed extensive cytoplasmic spreading and the formation of cellular networks over 28 days. In contrast, fibroblasts encapsulated in the physically incorporated gelatin hydrogels remained spheroidal. Hence, crosslinking ECM protein with synthetic matrix creates a stable scaffold with tunable mechanical properties and with long-term cell anchorage points, thus supporting cell attachment and growth in the 3D environment.
登录
查看更多内容
影响因子：
10.8
作者：
Burdick, JA;Mason, MN;Anseth, KS
通讯作者：
Anseth, KS
DOI：
10.1002/jbm.a.33106
发表时间：
2011-08
期刊：
Journal of biomedical materials research. Part A
影响因子：
--
作者：
Fu Y;Kao WJ
通讯作者：
Kao WJ
影响因子：
19
作者：
Brown, RA;Wiseman, M;Nazhat, SN
通讯作者：
Nazhat, SN
影响因子：
2.9
作者：
Miyawaki, O;Norimatsu, Y;Sakurai, H
通讯作者：
Sakurai, H
影响因子：
14
作者：
Liu, Yunxiao;Chan-Park, Mary B.
通讯作者：
Chan-Park, Mary B.