Comparison of the characteristics of human gingival fibroblasts and periodontal ligament cells.
Comparison of the characteristics of human gingival fibroblasts and periodontal ligament cells.
复制标题
人牙龈成纤维细胞和牙周膜细胞特征的比较。
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
H. Sugiya
中科院分区：
文献类型：
--
作者：
Y. Ogata;N. Niisato;T. Sakurai;S. Furuyama;H. Sugiya
为了阐明人类牙周韧带细胞的特性，我们将这些细胞与从女性受试者的牙周组织中分离的牙龈成纤维细胞进行了比较。与人牙龈成纤维细胞（HGF）相比，人牙周韧带（HPDL）细胞具有更清晰的纺锤形和更高的生长速度。HPDL细胞具有高水平的碱性磷酸酶（ALPase）活性，而HGF具有低水平的碱性磷酸酶活性。Northern blot分析显示HPDL细胞产生ALPase mRNA。在HPDL细胞和HGF中均检测到Decorin和biglycan mRNA，而骨钙素和骨唾液蛋白mRNA在两种细胞中均未检测到。HPDL细胞和HGF均对前列腺素E2 （PGE2）和异丙肾上腺素有应答，并产生环AMP (cAMP)，但对人1-34甲状旁腺激素（PTH）无应答。使用Fura 2-AM测量HPDL细胞和HGF中的细胞内Ca2+ ([Ca2+]i)。缓激素（BK）和组胺（HIS）是主要的化学介质，在细胞外Ca2+存在的情况下引起[Ca2+]i的短暂升高。在HGF而非HPDL细胞中，HIS诱导了[Ca2+]i的双相瞬时峰值。BK和HIS增加了HPDL细胞和HGF中PGE2的释放。然而，HGF比HPDL细胞释放更多的PGE2。这些结果表明HPDL细胞与HGF具有完全不同的特性。与HGF相比，HPDL细胞的增殖速率更高，cAMP的产生水平更高，ALPase活性更高，并且对化学介质（BK和HIS）的反应方式不同。
To elucidate the characteristics of human periodontal ligament cells, we compared these cells with gingival fibroblasts isolated from the periodontal tissues of female human subjects. Human periodontal ligament (HPDL) cells had a sharper spindle shape and exhibited a higher growth rate than human gingival fibroblasts (HGF). HPDL cells had a high level of alkaline phosphatase (ALPase) activity, whereas HGF had a low level of such activity. Northern blot analysis demonstrated that HPDL cells produced ALPase mRNA. Decorin and biglycan mRNA were detected in both HPDL cells and HGF, whereas osteocalcin and bone sialoprotein mRNA was not detected in either cells. Both HPDL cells and HGF responded to prostaglandin E2 (PGE2) and isoproterenol, and produced cyclic AMP (cAMP), but did not respond to human 1-34 parathyroid hormone (PTH). Intracellular Ca2+ ([Ca2+]i) was measured in HPDL cells and HGF, using Fura 2-AM. Bradykinin (BK) and histamine (HIS), which are major chemical mediators, caused a transient rise of [Ca2+]i in the presence of extracellular Ca2+. In HGF, but not HPDL cells, HIS induced a biphasic transient peak in [Ca2+]i. BK and HIS increased PGE2 release in both HPDL cells and HGF. However, HGF released a larger amount of PGE2 than HPDL cells. These results demonstrate that HPDL cells have quite different characteristics from HGF. HPDL cells proliferate at a higher rate than HGF, show higher levels of cAMP production and greater ALPase activity, and respond in a different fashion to chemical mediators (BK and HIS) compared with HGF.
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细胞内游离 Ca2 的增加与缓激肽存在下表皮生长因子对瑞士 3T3 成纤维细胞的无血清生长刺激有关。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Olsen,R;Santone,K;Melder,D;Oakes,SG;Abraham,R;Powis,G
通讯作者：
Powis,G
影响因子：
3
作者：
SOMERMAN, MJ;YOUNG, MF;SAUK, JJ
通讯作者：
SAUK, JJ
影响因子：
4.3
作者：
MARIOTTI, A;COCHRAN, DL
通讯作者：
COCHRAN, DL
影响因子：
4.3
作者：
MATSUDA, N;LIN, WL;GENCO, RJ
通讯作者：
GENCO, RJ
DOI：
10.1073/pnas.86.4.1143
发表时间：
1989-02-01
影响因子：
11.1
作者：
LIAN, J;STEWART, C;STEIN, G
通讯作者：
STEIN, G