Concentrated nanofat: a modified fat extraction promotes hair growth in mice via the stem cells and extracellular matrix components interaction.
Concentrated nanofat: a modified fat extraction promotes hair growth in mice via the stem cells and extracellular matrix components interaction.
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浓缩纳米脂肪：改良的脂肪提取通过干细胞和细胞外基质成分的相互作用促进小鼠毛发生长
DOI：
10.21037/atm-20-6086
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发表时间：
2020-09
影响因子：
--
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Li Z;Zhang J;Li M;Tang L;Liu H
背景脂肪移植似乎是治疗脱发的一种很有前途的细胞疗法。然而，吸脂剂中的杂质会削弱治疗效果。在此，我们发展了脂肪抽提物的提取方法，并在小鼠模型上研究了其促进毛发生长的作用和机制。方法将脂肪移植物制备成浓缩纳米脂肪(CNF)、去细胞CNF(DCNF)和脂肪来源干细胞(ADSCs)。将它们注射到脱毛小鼠背部皮下，以测试其生毛效果。将脂肪提取物的条件培养液(CM)应用于毛乳头细胞(DPC)，以检测细胞活力和生长期相关信号。结果CNF和高剂量的ADSCs可促进脱毛小鼠毛发生长，并诱导脱毛小鼠向生长期转化。DCNF和低剂量的ADSCs没有表现出这种作用，但当它们联合使用时，毛发生长得到促进。体外研究表明，与其他处理组相比，经CNF-CM处理的DPC具有更强的增殖、迁移、细胞周期进展和Wnt/β-catenin途径蛋白水平。结论CNF通过激活DPC和生长期诱导，具有比ADSCs更好的生发促进作用。在干细胞(SCs)和细胞外基质(ECM)的天然复合体中，ECM起着重要的补充作用，并放大ADSCs的能力。这些结果为CNF临床应用治疗脱发提供了理论依据。
Background Fat graft transplantation seems a promising cell therapy for hair loss. However, impurities in lipoaspirate weaken the treatment effect. Here, we developed the lipoaspirate extraction method then investigate the effect and mechanism on hair growth-promoting in a mouse model. Methods Fat graft was prepared into concentrated nanofat (CNF), decellularized CNF (DCNF), and adipose-derived stem cells (ADSCs). They were injected subcutaneously in the back of depilated mice to test the hair promoting effect. Conditioned media (CM) from the adipose extracts were applied to dermal papilla cells (DPCs) to evaluate the cell viability and the anagen related signal. Results CNF and a high dose of ADSCs promoted hair growth and induced telogen-to-anagen transition in depilated mice. DCNF and a low dose of ADSCs did not show such effect; however, hair growth was promoted when they were used in combination. In vitro study showed the CNF-CM treated DPCs exhibited increased proliferation, migration, cell cycle progression, and elevated Wnt/β-catenin pathway protein levels compared with the other treatment groups. Conclusions CNF has a better effect than ADSCs in hair promotion via activating the DPCs and anagen induction. In this nature complex of stem cells (SCs) and extracellular matrix (ECM), ECM serves a significant supplementary role and amplifies the power of ADSCs. These results supply a theoretical basis on the clinical application of CNF to treat hair loss.
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影响因子：
6.5
作者：
Kwack, Mi H.;Kim, Moon K.;Sung, Young K.
通讯作者：
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通讯作者：
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3.1
作者：
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通讯作者：
Schindeler, Aaron