In Vivo Bone Regeneration Capacity of Multiscale Porous Polycaprolactone-Based High Internal Phase Emulsion (PolyHIPE) Scaffolds in a Rat Calvarial Defect Model.
In Vivo Bone Regeneration Capacity of Multiscale Porous Polycaprolactone-Based High Internal Phase Emulsion (PolyHIPE) Scaffolds in a Rat Calvarial Defect Model.
复制标题
DOI：
10.1021/acsami.3c04362
复制
发表时间：
2023-06-14
影响因子：
9.5
通讯作者：
Claeyssens, Frederik
中科院分区：
文献类型：
--
作者：
Aldemir Dikici, Betul;Chen, Min-Chia;Dikici, Serkan;Chiu, Hsien-Chung;Claeyssens, Frederik
关键词：
在全球范围内，最常见的组织移植手术之一是骨移植。最近，我们报道了由光固化聚己内酯（4PCLMA）制成的聚合高内相乳液（PolyHIPES）的发展，并显示了其作为骨组织工程支架的体外潜力。然而，必须评估这些支架的体内性能，以临床上更相关的方式研究其潜力。因此，在这项研究中，我们的目的是比较大孔（使用立体光刻制造），微孔（使用乳液模板制造），和多尺度多孔（使用乳液模板和穿孔制造）支架的体内性能的4PCLMA。此外，由热塑性聚己内酯制成的3D打印大孔支架（使用熔融沉积成型制造）用作对照。将支架植入到临界尺寸的颅骨缺损中，在植入后4周或8周处死动物，并通过显微计算机断层扫描、牙科X线摄影和组织学评估新骨形成。与仅大孔或仅微孔支架相比，包括微孔和大孔的多尺度多孔支架在缺损区域中导致更高的骨再生。当比较一级多孔支架时，微孔支架在矿化骨体积和组织再生方面表现出比大孔支架更好的性能。Micro-CT结果显示，虽然大孔支架在第4周和第8周的骨体积/组织体积（Bv/Tv）值分别为8%和17%，但微孔支架的骨体积/组织体积（Bv/Tv）值明显更高，分别为26%和33%。总之，本研究报告的结果显示了多尺度PolyHIPE支架的潜在应用，特别是作为一种有前途的骨再生材料。
Globally, one of the most common tissue transplantation procedures is bone grafting. Lately, we have reported the development of polymerized high internal phase emulsions (PolyHIPEs) made of photocurable polycaprolactone (4PCLMA) and shown their potential to be used as bone tissue engineering scaffolds in vitro. However, it is essential to evaluate the in vivo performance of these scaffolds to investigate their potential in a clinically more relevant manner. Therefore, in this study, we aimed to compare in vivo performances of macroporous (fabricated using stereolithography), microporous (fabricated using emulsion templating), and multiscale porous (fabricated using emulsion templating and perforation) scaffolds made of 4PCLMA. Also, 3D-printed macroporous scaffolds (fabricated using fused deposition modeling) made of thermoplastic polycaprolactone were used as a control. Scaffolds were implanted into a critical-sized calvarial defect, animals were sacrificed 4 or 8 weeks after implantation, and the new bone formation was assessed by micro-computed tomography, dental radiography, and histology. Multiscale porous scaffolds that include both micro- and macropores resulted in higher bone regeneration in the defect area compared to only macroporous or only microporous scaffolds. When one-grade porous scaffolds were compared, microporous scaffolds showed better performance than macroporous scaffolds in terms of mineralized bone volume and tissue regeneration. Micro-CT results revealed that while bone volume/tissue volume (Bv/Tv) values were 8 and 17% at weeks 4 and 8 for macroporous scaffolds, they were significantly higher for microporous scaffolds, with values of 26 and 33%, respectively. Taken together, the results reported in this study showed the potential application of multiscale PolyHIPE scaffolds, in particular, as a promising material for bone regeneration.
登录
查看更多内容
影响因子：
5.5
作者：
Barbetta, A;Cameron, NR
通讯作者：
Cameron, NR
影响因子：
6.2
作者：
Christenson, Elizabeth M.;Soofi, Wafa;Mikos, Antonios G.
通讯作者：
Mikos, Antonios G.
影响因子：
6.2
作者：
Dikici, Betul Aldemir;Malayeri, Atra;Claeyssens, Frederik
通讯作者：
Claeyssens, Frederik
影响因子：
6.6
作者：
Dikici S;Aldemir Dikici B;MacNeil S;Claeyssens F
通讯作者：
Claeyssens F
影响因子：
6.2
作者：
Green, Brian J.;Worthington, Kristan S.;Guymon, C. Allan
通讯作者：
Guymon, C. Allan