Activation of the ubiquitin proteasome pathway by silk fibroin modified chitosan nanoparticles in hepatic cancer cells.
Activation of the ubiquitin proteasome pathway by silk fibroin modified chitosan nanoparticles in hepatic cancer cells.
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DOI：
10.3390/ijms16011657
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发表时间：
2015-01-12
影响因子：
5.6
通讯作者：
Tyan YC
中科院分区：
文献类型：
--
作者：
Yang MH;Chung TW;Lu YS;Chen YL;Tsai WC;Jong SB;Yuan SS;Liao PC;Lin PC;Tyan YC
丝素蛋白(SF)是一种具有巨大疏水结构域的蛋白质，可以很容易地提纯为无丝胶的丝素生物材料。丝素蛋白修饰壳聚糖纳米粒(SF-CSNP)是一种生物相容性材料，作为一种潜在的药物缓释系统得到了广泛的应用。本研究对肝细胞摄取SF-CSNP的生物学效应进行了研究。在本实验中，通过粒度分析和蛋白质分析对SF-CSNPs的特性进行了检测。SF-CSNP平均粒径为311.9±10.7 nm，Zeta电位为+13.33±0.3 mV。SF-CSNP的包覆量为6.27±0.17μg/mL。此外，利用蛋白质组学方法，通过分析HepG2细胞摄取SF-CSNP的差异蛋白表达，鉴定了参与泛素蛋白酶体途径的几个蛋白质。我们的实验结果表明，SF-CSNP可能参与了肝癌细胞的存活和增殖。
Silk fibroin (SF) is a protein with bulky hydrophobic domains and can be easily purified as sericin-free silk-based biomaterial. Silk fibroin modified chitosan nanoparticle (SF-CSNP), a biocompatible material, has been widely used as a potential drug delivery system. Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells. In this experiment, the characterizations of SF-CSNPs were measured by particle size analysis and protein assay. The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV. The SF coating on the SF-CSNP was 6.27 ± 0.17 μg/mL. Moreover, using proteomic approaches, several proteins involved in the ubiquitin proteasome pathway were identified by analysis of differential protein expressions of HepG2 cell uptake the SF-CSNP. Our experimental results have demonstrated that the SF-CSNP may be involved in liver cancer cell survival and proliferation.
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影响因子：
82.9
作者：
通讯作者：
--
影响因子：
1.9
作者：
Kasemo, B
通讯作者：
Kasemo, B
影响因子：
9.9
作者：
López-León, T;Carvalho, ELS;Bastos-González, D
通讯作者：
Bastos-González, D
影响因子：
3.7
作者：
Calvo, P;RemunanLopez, C;Alonso, MJ
通讯作者：
Alonso, MJ
影响因子：
--
作者：
Yang MH;Yuan SS;Chung TW;Jong SB;Lu CY;Tsai WC;Chen WC;Lin PC;Chiang PW;Tyan YC
通讯作者：
Tyan YC